Enzyme conjugate prepared with insoluble nonoparticle

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 5, 435 795, 436518, 436527, 436531, 436829, G01N 3353

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active

054057523

ABSTRACT:
The invention relates to a reagent consisting of at least one enzyme and at least one other substance, which are covalently or noncovalently bound to a particle, which is smaller than or is equal to 1000 Angstrom in diameter. The invention also relates to method and use of the reagent for determination or studies of a cell or a virus or another component in a sample. According to the invention, these determinations are made with for example some ELISA-technique (competitive, sandwich, etc), employing the reagent preferably in the form of a suspension in buffered water, instead of and in the same way as described for soluble enzyme conjugates. The substance can be antibody, a lectin, avidin or an antigen, the enzyme can be for example peroxidase, alcaline phosphatase, and the particle can be an inorganic or an organic polymeric compound or a combination thereof, as, for example, tresyl-activated glycerylpropyl-silica.

REFERENCES:
patent: 4267235 (1981-05-01), Rembaum et al.
patent: 4415665 (1983-11-01), Mosbach et al.
patent: 4434150 (1984-02-01), Azad et al.
patent: 4552839 (1985-11-01), Gould et al.
patent: 4698263 (1985-10-01), Wagner et al.
patent: 4918009 (1990-04-01), Nilsson
Nilsson et al., Methods in Enzymology 104: 56-69, 1984.

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