Enzyme compositions comprising a glucoamylase, an acid...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S183000, C435S203000, C435S205000, C435S252300, C435S320100, C536S023200

Reexamination Certificate

active

08048657

ABSTRACT:
The present invention relates to an enzyme blend composition comprising a glucoamylase, an acid stable alpha amylase, and an acid fungal protease. The present invention is further directed to a method for producing end products such as alcohols from fermentable sugars, comprising the steps of: (a) contacting a slurry comprising a milled grain that contains starch with an alpha amylase to produce a liquefact; (b) contacting the liquefact with a glucoamylase, an acid stable alpha amylase, and an acid fungal protease, to produce fermentable sugars; and (c) fermenting the fermentable sugars in the presence of a fermenting organism to produce end products.

REFERENCES:
patent: 4092434 (1978-05-01), Yoshizuma et al.
patent: 4514496 (1985-04-01), Yoshizumi et al.
patent: RE32153 (1986-05-01), Tamura et al.
patent: 4587215 (1986-05-01), Hirsh
patent: 4618579 (1986-10-01), Dwiggins
patent: 4760025 (1988-07-01), Estell et al.
patent: 5000000 (1991-03-01), Ingram et al.
patent: 5028539 (1991-07-01), Ingram et al.
patent: 5424202 (1995-06-01), Ingram et al.
patent: 5514583 (1996-05-01), Picataggio et al.
patent: 5554520 (1996-09-01), Fowler et al.
patent: 5612055 (1997-03-01), Bedford et al.
patent: 6352851 (2002-03-01), Nielsen et al.
patent: 7205138 (2007-04-01), Dunn-Coleman et al.
patent: 2006/0003408 (2006-01-01), Dunn-Coleman et al.
patent: 2006/0094080 (2006-05-01), Dunn-Coleman et al.
patent: WO 92/00381 (1992-01-01), None
patent: WO 92/06209 (1992-04-01), None
patent: WO 95/13362 (1995-05-01), None
patent: WO 97/20950 (1997-06-01), None
patent: WO 99/28488 (1999-06-01), None
patent: WO 00/04136 (2000-01-01), None
patent: WO 02/074895 (2002-09-01), None
patent: WO 2004/080923 (2004-09-01), None
patent: WO 2005/052148 (2005-06-01), None
patent: WO 2006/003408 (2006-01-01), None
patent: WO 2006/060062 (2006-06-01), None
patent: WO 2006/060062 (2006-06-01), None
patent: WO 2006/073839 (2006-07-01), None
patent: WO 2006/089107 (2006-08-01), None
Accession Q2WBH2, published Jan. 10, 2006.
Altschul, S.F. et al. “Local alignment statistics.”Methods Enzymol266: 460-480, 1993.
Altschul, Stephen F. et al., “Basic Local Alignment Search Tool,” J. Mol. Biol. 215:403-410, 1990.
Altschul, Stephen F. et al., “Gapped BLAST and PSI-BLAST: a new generation of protein database search programs,” Nucl. Acids Res., vol. 25, pp. 3389-3402, 1997.
Boel et al., “Glucoamylases G1 and G2 fromAspergillus nigerare synthesized from two different but closely related mRNAs,”The EMBO J., V. 3, N. 5, pp. 1097-1102, 1984.
Chen, H.M. et al. “Substitution of asparagine residues inAspergillus awamoriglucoamylase by site-directed mutagenesis to eliminate N-glycosylation and inactivation by deamidation,”Biochem. J., 301, (Pt 1):275-281 (1994).
Chen, H.M. et al. “Identification and elimination by site-directed mutagenesis of thermolabile aspartyl bonds inAspergillus awamoriglucoamylase,”Protein Eng., 8(6):575-582, (1995).
Chen, H.-M. et al. “Effect of replacing helical glycine residues with alanines on reversible and irreversible stability and production ofAspergillus awamoriglucoamylase,”Protein Eng., 9(6):499-505 (1996).
Dayhoff, M.O. et al., “A Model of Evolutionary Change in Proteins,” Atlas of Protein Sequence and Structure, National Biomedical Research Foundation, Washington, D.C., vol. 5, Supplement 3, Chapter 22, pp. 345-352 1978.
Hata, Y. et al. “The glucoamylase cDNA fromAspergillus oryzae: Its cloning, nucleotide sequence, and expression inSaccharomyces cerevisiae,” Agric. Biol. Chem., 55(4):941-949 (1991).
Jensen, B. et al. “Purification of extracellular amylolytic enzymes from the thermophilic fungusThermomyces lanuginosus,” Can. J. Microbiol., 34(3):218-223 (1988).
Miller, G.L. “Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar,”Anal. Chem., 31(3):426-428 (1959).
Needleman, S.B. et al. “A general method applicable to the search for similarities in the amino acid sequence of two proteins.”J. Mol. Biol48(3): 443-453, Mar. 1970.
Pearson, W.R. et al. “Improved Tools for Biological Sequence Comparison.”Proc. Natl. Acad. Sci. USA85(8): 2444-2448, Apr. 15, 1988.
Smith should be “Shpaer”, “GeneAssist—Smith Waterman and Other Database Similarity Searches and Identification of Motifs,”Methods in Molecular Biology, Sequence Data Anaylsis Guidebook, Humana Press, Inc. 70:173-187 (1997).
Smith, T.F. et al. “Comparison of biosequences.”Adv. Appl. Math2: 482-489, 1981.
Taylor et al., “Some properties of a glucoamylase produced by the thermophilic fungus humicola lanuginose,”Carbohydrate Research, 61 (1978) 301-308.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Enzyme compositions comprising a glucoamylase, an acid... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enzyme compositions comprising a glucoamylase, an acid..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzyme compositions comprising a glucoamylase, an acid... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-4307986

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.