Enzyme catalyzed template-independent creation of phosphodiester

Details Enzyme catalyzed template-independent creation of phosphodiester Enzyme catalyzed template-independent creation of phosphodiester

1994-09-02

1999-11-23

Wilson, James O.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 2531, 536 2532, 536 2533, 536 2534, C07H 100

Patent

active

059903007

ABSTRACT:
A method for the stepwise creation of phosphodiester bonds between desired nucleosides resulting in the synthesis of polynucleotides having a predetermined nucleotide sequence by preparing an initiation substrate containing a free and unmodified 3'-hydroxyl group; attaching a mononucleotide selected according to the order of the predetermined nucleotide sequence to the 3'-hydroxyl of the initiating substrate in a solution containing a catalytic amount of an enzyme capable of catalyzing the 5' to 3' phosphodiester linkage of the 5'-phosphate of the mononucleotide to the 3'-hydroxyl of the initiating substrate, wherein the mononucleotide contains a protected 3'-hydroxyl group, whereby the protected mononucleotide is covalently linked to the initiating substrate and further additions are hindered by the 3'-hydroxyl protecting group. Methods in which a mononucleotide immobilized on a solid support is added to a free polynucleotide chain are also disclosed.

REFERENCES:
patent: 4096324 (1978-06-01), Kelly et al.
patent: 4423212 (1983-12-01), Skulnick
patent: 4605735 (1986-08-01), Miyoshi et al.
patent: 4689405 (1987-08-01), Frank et al.
patent: 4719176 (1988-01-01), Klotz
patent: 4816571 (1989-03-01), Andrus et al.
patent: 4820812 (1989-04-01), Miyoshi et al.
patent: 4863849 (1989-09-01), Melamede
patent: 4876335 (1989-10-01), Yamane et al.
patent: 4948882 (1990-08-01), Ruth
patent: 4950745 (1990-08-01), Ishido et al.
patent: 4980460 (1990-12-01), Molko et al.
patent: 5003059 (1991-03-01), Brennan
patent: 5032680 (1991-07-01), Kawai et al.
patent: 5039796 (1991-08-01), Engels et al.
patent: 5047524 (1991-09-01), Andrus et al.
patent: 5091519 (1992-02-01), Cruickshank
patent: 5093232 (1992-03-01), Urdea et al.
patent: 5112963 (1992-05-01), Pieles et al.
patent: 5256549 (1993-10-01), Urdea et al.
patent: 5258506 (1993-11-01), Urdea et al.
patent: 5262530 (1993-11-01), Andrus et al.
patent: 5262536 (1993-11-01), Hobbs, Jr.
patent: 5264563 (1993-11-01), Huse
patent: 5264566 (1993-11-01), Froehler et al.
patent: 5268266 (1993-12-01), Fritsch et al.
patent: 5268464 (1993-12-01), Brill
patent: 5281701 (1994-01-01), Vinayak
patent: 5302509 (1994-04-01), Cheeseman
patent: 5348868 (1994-09-01), Reddy et al.
patent: 5362866 (1994-11-01), Arnold, Jr.
patent: 5367066 (1994-11-01), Urdeaa et al.
patent: 5380833 (1995-01-01), Urdea
patent: 5436143 (1995-07-01), Hyman et al.
Single-step elongation of oligodeoxynucleotides using terminal deoxynucleotidyl transferase, Herbert Schott and Herbert Schrade, Eur. J. Biochem. 143, 613-620 (1984).
Molecular Biology of Terminal transferase, Luch M.S. Chang, CRC Critical Reviews in Biochemistry, vol. 21, Issue 1.
Initiator role of double stranded DNA in terminal transferase catalyzed polymerization reactions, David J. Robbins and Mary Sue Coleman, Nucleic Acids Research, vol. 16, Nov. 7, 1988.
Hyman, Edward David The Hyman Method: Oligonucleotide Synthesis and Plasmid Preparation, (1995).
Sarfati et al., J. Biol. Chem., 265(31), 18902-18906 (1990).
Metzker et al., Nucleic Acids Res., 22(20), 4259-4267 (1994).
Canard et al., Proc. Nat'l. Acad. Sci. USA, 92, 10859-10863 (Nov. 1995).
Canard et al., Gene, 148, 1-6 (1994).
Kutateladze et al., FEBS, 207(2), 205-212 (1986).
Bollum, Fed. Proc. Soc. Exp. Biol. Med. 17, 193 (1958).
Deng and Wu, Meth. Enzymol., 100: 96-116 (1983).
Flugel et al., Biochem. Biophys. Acta., 308: 35-40 (1973).
Bennett et al., Biochemistry, vol. 12, No. 20 pp. 3956-3960 (1973).
England et al., Biochemistry, vol. 17, No. 11 pp. 2069-2076 (1978).
Kossel et al., Eur. J. Biochem, 22:271-276 (1971).
Kaufmann et al., Eur. J. Biochem, 24:4-11 (1971).
Hinton and Gumport, Nucleic Acids Res., 7:453-464 (1979).
Modak, Biochemistry, 17, 3116-3120 (1978).
Chang and Bollum, Biochemistry, vol. 10, No. 3 pp. 536-542 (1971).

Affiliated with

Also associated with

Rating

Enzyme catalyzed template-independent creation of phosphodiester does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enzyme catalyzed template-independent creation of phosphodiester, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzyme catalyzed template-independent creation of phosphodiester will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1223308