Enzyme-catalyzed chemiluminescent detection of proteins and nucl

Details Enzyme-catalyzed chemiluminescent detection of proteins and nucl Enzyme-catalyzed chemiluminescent detection of proteins and nucl

1992-12-23

1997-02-11

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

436501, 436172, 436819, C12Q 168

Patent

active

056019777

ABSTRACT:
A method and test kit using a hydroxyaryl cyclic diacylhydrazide, a peroxide and a peroxidase enzyme on a blotting membrane for detecting DNA, RNA or proteins (polypeptides) is described. The method and kit provides enhanced chemiluminescence in an assay because of the use of the membrane. The method and test kit is particularly useful in Western, Southern and Northern blotting type assays and DNA sequencing.

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