Enzyme-capture assay (ECA) for the identification of Escherichia

Details Enzyme-capture assay (ECA) for the identification of Escherichia Enzyme-capture assay (ECA) for the identification of Escherichia

1994-06-22

1997-03-18

Housel, James C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 74, 435 792, G01N 33569

Patent

active

056121865

ABSTRACT:
An enzyme-capture assay (ECA) for rapid identification of Escherichia coli (E. coli) in clinical samples comprising contacting a clinical sample which is suspected to contain E. coli with an immobilized antibody against .beta.-D-glucuronidase which then catalyzes an enzyme substrate to produce a fluorescent product is disclosed.

REFERENCES:
patent: 5268463 (1993-12-01), Jefferson
S.M. Holt et al., "Enzyme-Capture Assay for Rapid Detection of Escherichia coli in Oysters", Applied and Environmental Microbiology, Inc. vol. 55:229 (1989).
M.O. Husson et al., "Alkaline Phosphatase Capture Test for the Rapid Identification of Escherichia coli and Shigella Species Based on a Specific Monoclonal Antibody" Jour. of Clinical Microbiology, vol. 27:1518 (1989).
Shiu Wen Huang et al., "Enzyme Capture Assay for Rapid Identification of Escherichia coli in Blood Cultures", Journal of Clinical Microbiology, vol. 32:1444, (1994).
Shiu Wen Huang et al., "A Rapid Method for the Detection of Escherichia coli-Enzyme Capture Assay", (1993).
Frampton, E. W. et al. "Methods for Escherichia coli identification in food, water and clinical samples based on beta-glucuronidase detection." Journal of Applied Bacteriology, vol. 74 (1993), pp. 223-233.
Kasper, C. W. et al. "Coagglutination and Enzyme Capture Tests for Detection of Escherichic coli beta-galactosidase, beta-glucuronidase, and glutamate decarboxylase." Applied and Environmental Microbiology, vol. 53 (May 1987), pp. 1073-1077.

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