Enzyme-based regeneration of surface-attached nucleic acids

Details Enzyme-based regeneration of surface-attached nucleic acids Enzyme-based regeneration of surface-attached nucleic acids

2006-09-05

2006-09-05

Whisenant, Ethan (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C536S023100, C536S024300

Reexamination Certificate

active

07101669

ABSTRACT:
Enzyme-based regeneration of surface-attached nucleic acids is described herein. Microarrays involving hybridization of a probe to a target are important tools for genetic analysis. Conventionally, a microarray is used for a single analysis, after which it is discarded. The invention relates to a process for regeneration of a microarray through enzymatic digestion of a target from a surface-attached probe using a nuclease to digest a single strand of a nucleic acid duplex with directional specificity starting from the free end of the target strand. For example, a probe oligonucleotide bound to a gene chip at the 5′-end hybridizes to a target nucleic acid, leaving the 5′ end of the target open to 5′–3′ digestion. Lambda-exonuclease (λ-exonuclease) cleaves single nucleotides from the 5′ end of a duplex, progressing in the 5′–3′ direction. Once the target strand is digested, the enzyme is rinsed from the microarray. The microarray is thus regenerated and ready for a subsequent use.

REFERENCES:
patent: 4999284 (1991-03-01), Ward et al.
patent: 5374521 (1994-12-01), Kipling et al.
patent: 5501986 (1996-03-01), Ward et al.
patent: 5518900 (1996-05-01), Nikiforov et al.
patent: 5849486 (1998-12-01), Heller et al.
patent: 5880552 (1999-03-01), McGill et al.
patent: 6017696 (2000-01-01), Heller
patent: 6087112 (2000-07-01), Dale
patent: 6232066 (2001-05-01), Felder et al.
patent: 2003/0054388 (2003-03-01), Garner et al.
patent: 2004/0033525 (2004-02-01), Monforte et al.
patent: 2271179 (2000-11-01), None
patent: 0578138 (1994-01-01), None
patent: WO99/20640 (1999-04-01), None
patent: WO00/68419 (2000-11-01), None
Kazunori Okano, et al., DNA Probe Assay Based on Exonuclease III Digestion of Probes Hybridized on Target DNA,Analytical Biochemistry ,288, pp. 101-108, 1995, XP-002211015.
Russell G. Higuchi, et al, “Production of single-stranded DNA templates by exonuclease digestion following the polymerase chain reaction”,Nucleic Acids Research,17, Oct. 11, 1989, No. 19, XP-000226748.
Matthew Reason, et al., “Characterisation of fatty acid multilayers using a TSM biosensor”,Int. J. of Pharmaceutics,195, 2000, pp. 25-28, XP-002211016.
Francesco Ferrante, et al. “Molecular slip at the solid-liquid interface of an acoustic-wave sensor”,Journal of Applied Physics,76, No. 6, Sep. 15, 1994, pp. 3448-3462, XP-000470063.
Jorg D. Hoheisel, “On the Activities ofEscherichia coliExonuclease III”,Analytical Biochemistry,209, pp. 238-246, 1993, XP-002211017.

Affiliated with

Also associated with

Rating

Enzyme-based regeneration of surface-attached nucleic acids does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enzyme-based regeneration of surface-attached nucleic acids, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzyme-based regeneration of surface-attached nucleic acids will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3540830