Enzyme assays

Details Enzyme assays Enzyme assays

1979-06-29

1982-03-09

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 19, 435 20, 435 21, 435 22, 435 23, 435 24, 435288, 435299, 435300, 435805, 435806, 435810, C12Q 134

Patent

active

043189865

ABSTRACT:
An enzyme assay reagent comprises a substance which is capable of interaction with the enzyme to give rise to the formation of a compound of formula X--A--Y--NO.sub.2, wherein A comprises an aromatic nucleus, X comprises an auxochromic group and Y comprises an unsaturated group which is capable of transmitting electron resonance between the aromatic nucleus and the nitro substituent, said compound per se being capable of producing a visual signal which is easily discernible by eye.

REFERENCES:
patent: 3410756 (1968-11-01), Guibault et al.
patent: 3616251 (1971-10-01), Linoli
patent: 3875013 (1975-04-01), Manautou et al.
patent: 3968011 (1976-07-01), Manautou et al.
patent: 4039388 (1977-08-01), Gal et al.
patent: 4082781 (1978-04-01), Gal
patent: 4223090 (1980-09-01), Mazza
Chemical Abstracts, vol. 86, 166848h; 1977.
Dictionary of Organic Compounds, V. 3, pp. 1767-1768.
Dictionary of Organic Compounds, V. 4, p. 2469.
Dictionary of Organic Compounds, V. 4, p. 2471.

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