Enzyme assay using a solid phase substrate

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...

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435 19, 435 18, 435 15, C12Q 100, C12Q 144, C12Q 134, C12Q 148

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active

059725958

ABSTRACT:
This invention relates to a hydrophobic layer formed on a solid support, the hydrophobic layer incorporating by hydrophobic interactions an amphipathic enzyme substrate labeled with a reporter on its hydrophilic region, and the use of the same to conduct various enzyme assays without extraction steps.

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