Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1992-04-08
1995-07-25
Woodward, Michael P.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 28, 435962, 436 8, 436 34, 436825, C12Q 132, C12Q 128, G01N 3350, G01N 3352
Patent
active
054361338
ABSTRACT:
This invention relates to an enzyme assay for quantitative analysis of biochemical substances using NAD(P)-NAD(P)H system and utilizing hydrogen peroxide, in which isocitric acid, metal ions and isocitrate dehydrogenase are previously added to a test sample, thereby consuming the endogenous substances that interfere the analysis, and at the same time regenerating NAD(P)H reduced.
The isocitrate dehydrogenase is then inactivated by addition of a chelating agent, an enzyme or substrate that generates hydrogen peroxide as reaction product is added simultaneously or thereafter, and the amount of hydrogen peroxide thus formed is measured.
Biochemical substances that generate hydrogen peroxide as reaction product can be correctly analyzed by this method with no adverse effect of endogenous substances because these interfering substances have been removed prior to measurement.
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Fujita Tuyosi
Takagahara Isamu
Oriental Yeast Co. Ltd.
Woodward Michael P.
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