Enzyme assay and kit therefor

Details Enzyme assay and kit therefor Enzyme assay and kit therefor

1979-12-26

1981-07-14

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving luciferase

435 14, 435 15, 435 18, 435 21, 435175, 435176, 435177, 435178, 435179, 435180, 435181, 435182, C12Q 16, C12N 1118, C12N 1114, C12N 1102

Patent

active

042787610

ABSTRACT:
Compositions and methods featuring, in one aspect, an enzyme assay in which one of a pair of enzymes is immobilized on an insoluble support, and brought into contact with a solution containing the other enzyme of the pair and the substrates of both enzymes. The enzymes catalyze reactions between the substrates, and one or more products of the last such reaction are measured.

REFERENCES:
Jablonski et al., Proc. Natl. Acad. Sci., vol. 73, pp. 3848-3851, 1976.
Mosbach et al., Methods In Enzymology, ed. Mosbach, vol. 44, pp. 453-478, Academic Press, 1976.
Oskar Zaborsky, Immobilized Enzymes, pp. 124-126, Chemical Rubber Company Press, 1973.
Lemuel B. Wingard, Jr. et al., Applied Biochemistry and Bioengineering, vol. 1, Immobilized Enzyme Principles, pp. 204-211, 1976.
Ichiro Chibata, Editor, Immobilized Enzymes, pp. 218-219, A Halstead Press Book, John Wiley & Sons, 1978.

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