Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1990-11-13
1993-11-16
Yarbrough, Amelia Burgess
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 21, 435 25, 435 28, 435968, 435 74, 435 791, 436 56, 436501, 436512, 436546, 436800, 536 231, 935 76, 935 77, 935 98, 935 81, C12G 168
Patent
active
052622991
ABSTRACT:
A method and compounds useful for this method are described for enzyme-amplified signal detection in analytical assays requiring extremely high detection sensitivity which uses a substrate capable of being transformed by an enzyme from a compound which does not form a luminescent lanthanide chelate into a product which forms a luminescent lanthanide chelate. The method in which a substrate not capable of forming a highly luminescent lanthanide chelate is enzymatically altered to produce a product which forms a highly luminescent lanthanide chelate and hence is particularly useful in time-resolved luminescence analysis as required in many different heterogeneous or homogeneous assay formats is described herein.
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Evangelista Ramon A.
Pollak Alfred
Templeton Eva F. G.
Burgess Yarbrough Amelia
Kronem Systems, Inc.
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