Enzyme-aminoglycoside conjugates

Details Enzyme-aminoglycoside conjugates Enzyme-aminoglycoside conjugates

1980-02-28

1982-05-04

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Stablizing an enzyme by forming a mixture, an adduct or a...

435177, 260112R, C12N 996

Patent

active

043283110

ABSTRACT:
Methods and compositions are provided for conjugating a wide variety of compounds, particularly polyfunctional compounds, having a mercapto group, either naturally present or synthetically introduced, to a polyamino compound, particularly a polypeptide (including proteins). The method employs a haloalkyl-carbonyl compound, which is conjugated to one or more of the amino groups under mild acylating conditions. This is followed by combining the acylated polyamino compound with a mercapto containing compound, whereby the halogen is displaced by the sulfur of the mercapto group to form a stable thioether linkage. The resulting conjugates, depending on the compounds involved, can find uses in immunoassays, as hapten-antigen conjugates for the production of antibodies, and as ligand analog enzyme conjugates for use as reagents in controlling the distribution of substitution of a mercapto compound to a polyamino compound.

REFERENCES:
patent: 4039385 (1977-08-01), Ulman et al.
patent: 4067774 (1978-01-01), Rubenstein et al.
patent: 4100268 (1978-07-01), Scherr
patent: 4144131 (1979-03-01), Richardson

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