Enzyme activation protease assay

Details Enzyme activation protease assay Enzyme activation protease assay

2007-08-14

2007-08-14

Weber, Jon (Department: 1657)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

Reexamination Certificate

active

10353908

ABSTRACT:
A protein reagent is provided for measuring protease enzyme activity in a sample. The protein reagent comprises two inhibition entities joined by a linker comprised of an indicator enzyme donor and an amino acid sequence susceptible to enzymatic cleavage. The protein reagent is substantially inhibited from binding to the cognate enzyme acceptor fragment, while the product of the enzymatic cleavage binds to the cognate enzyme acceptor fragment to form a functional indicator enzyme. The indicator enzyme activity is related to the protease enzyme activity of the sample.

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Eglen, R. M., Assay &Drug Dev. Tech., vol. 1(1), pp. 97-104, (Nov. 2002)(Abstract Only).
Webster's II New Riverside University Dictionary, p. 946 (1994).
HitHunter Enzyme Fragment Complementation, Cyclic-AMP Assay Kit DaTA Sheet, (Copyright 2001).
Eglen, 2002.ASSAY and Drug Development Technologies, vol. 1, No. 1, pp. 97-104.

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