Enzymatically inactive, immunologically-active .beta.-galactosid

Details Enzymatically inactive, immunologically-active .beta.-galactosid Enzymatically inactive, immunologically-active .beta.-galactosid

1988-07-12

1990-10-16

Weimer, Elizabeth C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 14, 435 18, 4351721, 435181, 435188, 435207, 436512, 436532, 436537, 530388, 530402, G01N 3353, G01N 33563, C07K 300

Patent

active

049634690

ABSTRACT:
The present invention provides an enzymatically-inactive, immunologically-active .beta.-galactosidase mutein, wherein, in the region between the amino acids 430 and 550, at least one amino acid of the natural sequence is changed to another amino acid and the enzymatic activity does not amount to more than 1%, referred to the native enzyme. The present invention also provides a process for the production of this mutein. Furthermore, the present invention is concerned with the use of this mutein in the immunological determination of serum proteins by the enzyme immunoassay principle.

REFERENCES:
patent: 4446233 (1984-05-01), Auditore-Hargreaves et al.
patent: 4668620 (1987-05-01), Armenta et al.
patent: 4810635 (1989-03-01), Ledden et al.
Ring et al. "Site-Directed Matagenesis of .beta.-Galactosidase . . . ", Biochem. Biophys. Res. Comm. 152(3), 1050-5(5/1988).
Fowler et al., "Methionine 500, the Site of Covalent Attachment . . . .beta.-Galactosidase", J. Biol. Chem. 253(190), 5283-5285(8/10/78).

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