Enzymatic urea assay

Details Enzymatic urea assay Enzymatic urea assay

1984-06-08

1986-08-26

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving urea or urease

435 15, 435 25, C12Q 158, C12Q 148, C12Q 126

Patent

active

046083359

ABSTRACT:
A composition, device and method useful as an enzymatic urea assay based on the use of urea amidolyase comprising urea amidolyase, pyruvate kinase, pyruvate oxidase, mono and divalent cations, phosphoenolpyruvate, thyamine pyrophosphate, ATP, bicarbonate, phosphate, a color indicator system and optionally inorganic phosphate, a buffer having a pH range of from about 6.5 to 9.5, and sodium or potassium ferrocyanide. After contacting the test solution or body fluid sample with the assay composition, reading can be accomplished visually or instrumentally.

REFERENCES:
patent: 3655516 (1972-04-01), Roon et al.
patent: 4059407 (1977-11-01), Hochstrasser
patent: 4248973 (1981-02-01), Kallies
Colowick, et al., Methods in Enzymology, vol. I, (1955), pp. 482-486, Acedemic Press, Inc.
Tabor, et al., Methods in Enzymology, vol. XVIIB, (1971), pp. 741-746, Acedemic Press Inc.

Affiliated with

Also associated with

Rating

Enzymatic urea assay does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enzymatic urea assay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzymatic urea assay will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2312449