Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Reexamination Certificate
2006-02-14
2006-02-14
Meller, Michael (Department: 1654)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
C435S068100, C514S002600
Reexamination Certificate
active
06998259
ABSTRACT:
Enzymatic digests of whey protein concentrates were prepared using animal, bacterial and fungal proteases, and evaluated for antihypertensive activities. The highest ACE-inhibitory activity was obtained with the purified peptide β-1g (f142–148) obtained by chemical synthesis, for which an IC50value of 0.04 mg powder.ml−1was found. The hydrolysates derived from BiPRO™ whey protein isolate and β-1g both gave higher antihypertensive activities (IC50values of 0.29 to 0.90 mg powder.ml−1) than the other hydrolysates tested (IC50values of 0.96 and 1.30 mg powder.ml−1). The recovered hydrolysate can be used to treat hypertension in mammals such as humans and domestic pets such as dogs and cats.
REFERENCES:
patent: 3857966 (1974-12-01), Feldman et al.
patent: 3932672 (1976-01-01), Pour-El et al.
patent: 3970520 (1976-07-01), Feldman et al.
patent: 4001437 (1977-01-01), Jaeggi et al.
patent: 4107334 (1978-08-01), Jolly
patent: 4154675 (1979-05-01), Jewett et al.
patent: 4218490 (1980-08-01), Phillips et al.
patent: 4293571 (1981-10-01), Olofsson et al.
patent: 4293583 (1981-10-01), Farr et al.
patent: 4427658 (1984-01-01), Maubois et al.
patent: 4482574 (1984-11-01), Lee
patent: 4486413 (1984-12-01), Wiesenberger et al.
patent: 4847096 (1989-07-01), Mellqvist et al.
patent: 4981704 (1991-01-01), Thibault
patent: 5039532 (1991-08-01), Jost et al.
patent: 5322773 (1994-06-01), Kaneko et al.
patent: 5369015 (1994-11-01), Yoshikawa et al.
patent: 5405637 (1995-04-01), Martinez et al.
patent: 5589357 (1996-12-01), Martinez et al.
patent: 5695796 (1997-12-01), Yamamoto et al.
patent: 5744179 (1998-04-01), Shimamura et al.
patent: 5854029 (1998-12-01), Yamamoto
patent: 5869444 (1999-02-01), Klein
patent: 5882705 (1999-03-01), Sato et al.
patent: 5952193 (1999-09-01), Shimamura et al.
patent: 6630320 (2003-10-01), Davis et al.
patent: 0 474 506 (1998-05-01), None
patent: 04082898 (1992-03-01), None
patent: 04282398 (1992-10-01), None
patent: 04282400 (1992-10-01), None
patent: 06345664 (1994-12-01), None
patent: 08269088 (1996-10-01), None
patent: 99/65326 (1999-12-01), None
Hawley's Condensed Chemical Dictionary, Lewis, Sr., 13thEdition, 1997, p. 937.
Abubakar, A. et al; “New Derivation of the Inhibitory Activity Against Angiotensin Converting Enzyme (ACE) from Sweet Cheese Whey”;Tohoku J. Agri. Res.vol. 47 No. 1-2, Sep. 1-8, 1996.
Abubakar, A. et al; “Structural analysis of New Antihypertensive Peptides Derived from Cheese Whey Protein by Proteinase K Digestion”;J. Dairy Sci.81:12, 3131-3138; 1996.
Cushman, D. W. et al; “Spectrophotometric Assay and Properties of the Angiotensin-Converting Enzyme of Rabbit Lung”;Biochem Pharmacol20: 163 7-1648; 1971.
Ferreira, S.H., et al; “Isolation of Bradykinin-Pptentiating Peotides fromBothropsjararaca Venom”;Biochemistry, vol. 9, No. 13; 1970.
Kohmura, M., et al; “Inhibition of Angiotensin-converting Enzyme by Synthetic Peptides of Human β-Casein”,Agri. Biol. Chem.53(8). 2107-2114; 1989.
Maruyama, S., et al.; “A Peptide Inhibitor of Agiostensin I Converting Enzyme in the Tryptic Hydrolysate of Casein”;Agri. Biol. Chem.46(5); 1393-1394, 1982.
Maubois, J.L., et al; “Les peptides du lait à{grave over ( )} activité physiologique III. Peptides du lait è effet cardiovasculaire: activités antithrombotique et antihypertensive”,Lait, 71:249-255.
Mullally, M. M., et al; “Identification of a novel angiotensin-I-converting enzyme inhibitory peptide corresponding to a tryptic fragment of bovine β-lactoglobulin”;FEBS Letters, 402:99-101.
Oshima, G., et al; Peptide Inhibitors of Angiotension-I-Converting Enzyme in Digests of Gelatin by Bacterial Collagenase;Biochimica et Biophysica Acta566, 128-137, 1979.
Pearce R. J.; “Whey Protein Recovery and Whey Protein Fractionation”;Whey and Lactose Processing; J.G. Zadow, ed., Elsevier, 271-316, London.
Takano, Toshiaki; “Milk Derived Peptides and Hypertension Reduction”;Int. Dairy Journal8 (1980) 375-381; Great Britain.
“Antihypertensive Effects of Whey Protein Hydrolysates in Rats Reduces Mean Arterial Blood Pressure”,Davisco Press Release, (2000).
“Davisco Foods International, Advitech Solutions Join Forces to Produce Whey Protein Hydrolysates”,Davisco Press Release, (1998).
“Davisco Nominated for Prestigious International Award for its Patented Hypertension-Reducing Whey Protein”,Davisco Press Release, (1999).
Ju et al., “Effects of Limited Proteolysis on Gelation and Gel Properties of Whey Protein Isolate”,Journal of Dairy Science, 78, 2119-2128 (1995).
Laragh, “L'hypertension”,La Recherche, 10, 1068-1076 (1979). (abstract).
Otte et al., “Effects of Limited Proteolysis on the Microstructure of Heat-Induced Whey Protein Gels at Varying pH”,Journal of Dairy Science, 79, 782-790 (1996).
Allain Anne-Francoise
Davis Martin E.
Gauthier Sylvie
Gourley Line
Pouliot Yves
Davisco Foods International
Meller Michael
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