Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing organic compound containing a metal or atom other...
Patent
1991-05-01
1993-02-23
Robinson, Douglas W.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing organic compound containing a metal or atom other...
435196, 435128, 558105, C12P 900, C12P 1300, C12N 916, C07F 902
Patent
active
051889510
ABSTRACT:
A method of preparing a soluble monovalent salt of a phosphatidyl ester which comprises reacting a phospholipid with a primary alcohol in the presence of an enzyme catalyst in a divalent cationic buffered solution and a water immiscible non-ether solvent that does not inactivate the enzyme, to form a divalent cationic salt of the phosphatidyl ester, and suspending the product in the presence of a stoichiometric amount of a monovalent cationic salt whose anion forms an insoluble salt with the divalent cation. The use of Centrifugal Partition Chromatography facilitates the enzyme reaction. The monovalent salt is preferably an ammonium/sodium mixed salt.
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Marziani Frank
Pilkiewicz Frank G.
Tino John A. F.
Tremblay Paul-Alain
Bloom Allen
Janofsky Ilene
Robinson Douglas W.
The Liposome Company Inc.
Weber Susan M.
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