Enzymatic synthesis of (S)3-hydroxy-3-methylglutaryl coenzyme A

Details Enzymatic synthesis of (S)3-hydroxy-3-methylglutaryl coenzyme A Enzymatic synthesis of (S)3-hydroxy-3-methylglutaryl coenzyme A

1984-06-04

1987-05-12

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing sulfur-containing organic compound

435195, 435874, C12N 918, C12P 1100, C12R 138

Patent

active

046650261

ABSTRACT:
A novel method for the production of (S)3-hydroxy-3-methylglutaryl coenzyme A provided for the enzymatic conversion of 3-methylcrotonyl coenzyme A to the desired product. The enzyme is obtained from Pseudomonas citronellolis by ammonium sulfate precipitation of a cellular lysate. The precipitate is then redissolved in a suitable buffer and yields of over 60% have been obtained by direct reaction in the buffer.

REFERENCES:
Rilling et al.; "The Enzymatic Isomerization of .beta.-Methylvinylacetyl Coenzyme A and the Specificity of a Bacterial .beta.-Methylcrotonyl Coenzyme A Carboxylase"; J. Biol. Chem., vol. 235, No. 11, 1960, pp. 3087-3092.
Coon, M. J.; "Enzymes of Isovaleryl CoA Metabolism"; Methods in Enzymology, vol. V; 1962; pp. 896-899.
Lehninger, "Biochemistry", Worth Publishing Co., 1970, p. 443.
U. Henning, (1959), Archives of Biochemistry and Biophysics, 83:259-267.
R. Ray Fall, (1981), Methods in Enzymology 71:791-799.

Affiliated with

Also associated with

Rating

Enzymatic synthesis of (S)3-hydroxy-3-methylglutaryl coenzyme A does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enzymatic synthesis of (S)3-hydroxy-3-methylglutaryl coenzyme A, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzymatic synthesis of (S)3-hydroxy-3-methylglutaryl coenzyme A will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1802406