Enzymatic synthesis of phosphorothioate oligonucleotides using r

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 5, 435 6, 435 911, 435 913, 435 915, 435 912, 536 221, 536 231, 536 243, 536 2431, C07H 2100, C07H 2104, C12Q 168, C12P 1934

Patent

active

057393117

ABSTRACT:
The present invention relates to methods of synthesizing phosphorothioate oligonucleotides. In particular, it relates to the use of certain restriction endonucleases to cleave phosphorothioate oligonucleotides which contain restriction endonuclease recognition sequences. These restriction sequences facilitate the cleavage of relatively cleavage resistant phosphorothioate oligonucleotides thus facilitating their separation and purification after synthesis.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4786600 (1988-11-01), Kramer et al.
patent: 4800159 (1989-01-01), Mullis et al.
patent: 4851330 (1989-07-01), Kohne
patent: 4994368 (1991-02-01), Goodman et al.
patent: 5030557 (1991-07-01), Hogan et al.
patent: 5212295 (1993-05-01), Cook
patent: 5264423 (1993-11-01), Cohen et al.
patent: 5276019 (1994-01-01), Cohen et al.
patent: 5286717 (1994-02-01), Cohen et al.
patent: 5288611 (1994-02-01), Kohne
patent: 5418419 (1995-05-01), Gelfand et al.
patent: 5436143 (1995-07-01), Hyman
patent: 5470967 (1995-11-01), Huie et al.
Olsen, et al., "Interaction of Restriction Endonucleases with Phosphorothioate-Containing DNA", Nucleosides & Nucleotides, 10(1-3):665-667, (1991).
Vosberg & Eckstein, "Effect of Deoxynucleoside Phosphorothioates Incorporated in DNA on Cleavage By Restriction Enzymes", J. Biol. Chem., 257:6595-6599, (1982).
Potter et al, "Cleavage of phosphorothioate-substituted DNA by restriction endonucleases", J. Biol. Chem. 259(23):14243-14248, Nov. 1984.
Bartlett, et al. Stereochemical course of polymerization catalyzed by avian myeloblastosis virus reverse transcriptase. The Journal of Biological Chemistry. 257:8879-8884. U.S.A. (1982).
Bergot, et al. The manufacture and molecular characterization of phosphorothioate deoxynucleotides for use as antisense therapeutics in human clinical trials. Nucleic Acids Symposium Series. 29:57. Oxford University Press, Oxford, U.K. (1993).
Biggin, et al. Buffer gradient gels and S label as an aid to rapid DNA sequence determination. Proc. Nat.l Acad. Sci. USA. 80:3963-3965. Biochemistry, (1983).
Brody, et al. Stereochemical course of nucleotidyl transfer catalyzed by bacteriophage T7 induced DNA polymerase. Biochemistry. 21:2570-2572. (1982).
Brown, T., et al. Modern machine-aided methods of oligodeoxyribonucleotidesynthesis. Oligonucleotides and Analogues: A Practical Approach . 1-25. IRL Press, Oxford, U.K. (1991).
Burgers, et al. A study of the mechanism of DNA polymerase I from escherichia coli with diasteromeric phosphorothioate analogs of deoxyadenosine triphosphate. The Journal of Biological Chemistry. 254:6889-6893. U.S.A. (1979).
Dyatkina, et al. Nucleic Acids Research. 238. Oxford University Press, Oxford, England (1991).
Eckstein, et al. Nucleotide Phosphorothioates. 54:367-402. Ann. Rev. Biochem., (1985).
Leiter, et al. Inhibition of influenza virus replication by phosphorothioate oligodeoxynucleotides. Proc. Nat'l Acad. Sci. USA. 87:3430-3434. Microbiology, (1990).
Matsukara, et al. Synthesis of phosphorothioate analogues of oligodeoxyribonucleotides and their antiviral activity against human immunodeficiency virus (HIV). Gene 72:343-347. (1988).
McGilvery, R., et al., Changes in the Genetic Message. Biochemistry. A functional approach. 3:125-126. W.B. Saunders Company, Philadelphia, London, Toronto, Mexico City, Rio de Janeiro, Syndey, Tokyo, (1983).
Mullis, et al. Specific synthesis of DNA in vitro via a. polymerase-catalyzed chain reaction. Methods in Enzymology. 155:335-350. Academy Press, Inc. (1987).
Murakawa, et al. Laboratory Methods. Direct detection of HIV-1 RNA from AIDS and ARC patient samples. DNA. 7:287-295. Mary Ann Liebert, Inc., Publishers (1988).
Romaniuk, et al. A study of the mechanism of T4 DNA polymerase with diaseromeric phosphorothioate analogues of deoxyadenosine triphosphate. The Journal of Biological Chemistry. 257:7684-7688. U.S.A. (1982).
Sinha, D. Large-Scale oligonucleotide synthesis using the solid-phase approach. Methods in Molecular Biology: Protocols for Oligonucleotides and Analogs. 20:437-463. Humana Prss Inc., Totowa, N.J. (1993).
Stec, W., et al. Novel route to oligo(deoxyribonucleotide phosphorothioates). Stereocontrolled synthesis of P-chiral oligo(deoxyribonucleotide phosphorothioates). Nucleic Acids Research. 19:5883-5888. Oxford University Press, Oxford, U.K. (1991).
Stec, W., et al. Automated solid-phase synthesis, separation, and stereochemistry of phosphorothioate analogues of oligodeoxyribonucleotides. J. Am. Chem. Soc. 106:6077-6079. American Chemical Society, (1984).
Taylor, et al. The use of phosphorotioate-modified DNa in restriction enzyme reactions to prepare nicked DNA. Nucleic Acids Research. 13:8749-8764. IRL Press Limited, Oxford, England (1985).
Degradation and modification of nucleic acids. Chapter 4, Biochemistry of the Nucleic Acids. 97-108, (Adams, et al., ed. 11th ed. 1992).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Enzymatic synthesis of phosphorothioate oligonucleotides using r does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enzymatic synthesis of phosphorothioate oligonucleotides using r, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzymatic synthesis of phosphorothioate oligonucleotides using r will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-636089

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.