Enzymatic synthesis of oligonucleotides using 3'-ribonucleotide

Details Enzymatic synthesis of oligonucleotides using 3'-ribonucleotide Enzymatic synthesis of oligonucleotides using 3'-ribonucleotide

1997-10-10

1999-06-29

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435 912, 514 44, 536 243, 536 2433, 536 253, C12Q 168, C12P 1934, A01N 4304, C07H 2104

Patent

active

059167777

ABSTRACT:
The present invention relates to methods of enzymatically synthesizing oligonucleotides. In particular, it relates to the use of 3'-ribonucleotide primers which effectively serve to initiate oligonucleotide synthesis, and can also be easily cleaved from synthesis products and reused in subsequent synthesis reactions.

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