Enzymatic synthesis of oligonucleotides

Details Enzymatic synthesis of oligonucleotides Enzymatic synthesis of oligonucleotides

1994-02-23

1997-07-08

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 935 77, 935 78, C12P 1934, C12Q 168

Patent

active

056459874

ABSTRACT:
The present invention makes possible the catalytic production of sequence specific oligonucleotides through the use of a specially designed template sequence. The reaction can be made to proceed isothermally in the presence of an excess of nucleoside triphosphates, an agent for polymerization, and a cutting agent. Because the process is catalytic with respect to the template sequence, an unlimited amount of oligonucleotide product can theoretically be generated from a single molecule of template. Where the process is initiated by the presence of a nucleic acid target sequence, the method of the present invention can be used for diagnostic purposes as an amplification method to improve sensitivity. Diagnostic sensitivity can be further enhanced by employing a cascade of these template sequences.

REFERENCES:
patent: 4994368 (1991-02-01), Goodman et al.

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