Enzymatic substrates for detecting Pseudomonas aeruginas

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S024000

Reexamination Certificate

active

06905841

ABSTRACT:
An enzyme substrate which includes a target portion and a marker portion, hydrolysis of the substrate leading to separation of the target portion from the marker portion, and the target portion being specific for the enzyme activity being assayed. A formulation containing at least one such substrate, and a method for detecting the bacterial speciesPseudomonas aeruginosais also disclosed. The target portion of the substrate is specific for β-alanine aminopeptidase activity and the marker portion is a compound which reveals whether a hydrolysis reaction has taken place or not. The invention is particularly applicable in the field of diagnosis.

REFERENCES:
patent: 5571684 (1996-11-01), Lawrence et al.
patent: 5610029 (1997-03-01), Ehrenfeld et al.
patent: 6046016 (2000-04-01), Orenga
patent: 6472167 (2002-10-01), Townsend et al.
patent: WO 94/21816 (1994-09-01), None
patent: WO 99 51767 (1999-10-01), None
Kasafirek,, et al., “Role of Amino Acid Residues in Chromogenic Substrates Cleaved by Pancreatic Elastase,” 52Collect. Czech. Chem. Commun. 1625-33 (1987).
Suszkiw, et al., “Brain Aminoacyl Arylamidase. Further Purification of the Soluble Bovine Enzyme and Studies on Substrate Specificity and Possible Active-Site Residues,” 9Biochemistry, 4008-4017 (1970).
Vermelho , et al., “Detection of Extracellular Proteases From Microorganisms on Agar Plates,” 91Memorias Do Instituto Oswaldo Cruz755-760 (1996).
Louis et al., “Spectrophotometric Assay For Amidolytic Activity of Alkaline Protease FromPseudomonas Aeruginosa, ” 345Analytica Chimica Acta219-225 (1997).
Besson et al., “Synthetic Peptide Substrates For a Conductimetric Assay ofPseudomonas AeruginosaElastase,” 237Analytical Biochemisty216-223 (1996).
Saulnier et al., “Conductimetric Assay of Elastase in the Supernatants of Cultures ofPseudomonas-AeruginosaStrains,” 247Analytica Chimica Acta79-82 (1991).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Enzymatic substrates for detecting Pseudomonas aeruginas does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enzymatic substrates for detecting Pseudomonas aeruginas, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzymatic substrates for detecting Pseudomonas aeruginas will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3465523

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.