Chemistry: molecular biology and microbiology – Process of utilizing an enzyme or micro-organism to destroy... – Resolution of optical isomers or purification of organic...
Patent
1993-06-25
1996-02-20
Wax, Robert A.
Chemistry: molecular biology and microbiology
Process of utilizing an enzyme or micro-organism to destroy...
Resolution of optical isomers or purification of organic...
435 41, 435135, 435212, C12N 948, C12P 762, C12P 4100
Patent
active
054928303
ABSTRACT:
A method for the resolution of .alpha.-tertiary carboxylic acid esters by partial hydrolysis is disclosed. The partial hydrolysis is carried out by contacting the esters with an aqueous solution in the presence of a serine carboxypeptidase, and the hydrolysis product is separated from unreacted starting material to obtain the hydrolysis product or the unreacted starting material in enantiomerically enriched form. A novel ester hydrolase which is particularly useful in this method, and a nucleotide sequence encoding this enzyme, are also described.
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Blythe Todd
Kalwass Helmut
Rogers Elizabeth
Shames Spencer
Yee Christopher
Genzyme Corporation
Gosz William G.
Grimes Eric
Wax Robert A.
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