Enzymatic removal of a protein amino-terminal sequence

Details Enzymatic removal of a protein amino-terminal sequence Enzymatic removal of a protein amino-terminal sequence

1989-05-09

1992-06-30

Doll, John

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435 694, 530303, C12P 2106, C12N 1517, C07K 740

Patent

active

051262495

ABSTRACT:
This disclosure relates to new compounds and to a process for the controlled conversion of such compounds to produce a Protein that comprises an amino acid sequence which is useful as a precursor to human insulin or to a modified human insulin, the amino terminus portion of which sequence does not define a cathepsin C dipeptide removal stop point. The compounds have the formula Met-Y-Protein in which Y is Tyr or Arg.

REFERENCES:
Hsiung et al. Expression of Bovine Growth Hormone Derivatives in Escherischia coli and the use of the Derivatives Method in Enzymology, vol. 153, pp. 390-401 1987. . .
McDonald et al., Preparation and Specificity of Dipeptidyl Aminopeptidase I Methods in Enzymology, vol. 25, pp. 272-281 1972.
J. K. McDonald et al., Tissue Proteinases, A. J. Barrett and J. T. Dingles, Eds., North-Holland Publishing Co., Amsterdam, 69-107 (1971).
J. K. McDonald et al., Proteinases in Mammalian Cells and Tissues, A. J. Barrett, Ed., Elsevier/North-Holland Biomedical Press, New York, 311-322 (1977).
J. K. McDonald et al., Methods in Enzymology, 25, C. H. W. Hirs and S. N. Timasheff, Eds., Academic Press, New York, 272-281 (1972).

Affiliated with

Also associated with

Rating

Enzymatic removal of a protein amino-terminal sequence does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enzymatic removal of a protein amino-terminal sequence, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzymatic removal of a protein amino-terminal sequence will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1863257