Enzymatic removal of a protein amino-terminal sequence

Details Enzymatic removal of a protein amino-terminal sequence Enzymatic removal of a protein amino-terminal sequence

1992-04-06

1994-10-04

Elliott, George C.

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

Insulin; related peptides

530305, 435 681, C07K 740, A61K 3726

Patent

active

053527690

ABSTRACT:
This disclosure relates to new compounds and to a process for the controlled conversion of such compounds to produce a Protein that comprises an amino acid sequence which is useful as a precursor to human insulin or to a modified human insulin, the amino terminus portion of which sequence does not define a cathepsin C dipeptide removal stop point. The compounds have the formula Met-Y-Protein in which Y is Tyr or Arg.

REFERENCES:
patent: 4946828 (1990-08-01), Markussen
patent: 5164366 (1992-11-01), Balschmidt et al.
J. K. McDonald et al., Tissue Proteinases, A. J. Barrett and J. T. Dingles, Eds., North-Holland Publishing Co., Amsterdam, 69-107 (1971).
J. K. McDonald et al., Proteinases in Mannalian Cells and Tissues, A. J. Barrett, Ed. Elsevier/North-Holland Biomedical Press, New York, 311-322 (1977).
J. K. McDonald et al., Methods in Enzymology, 25, C. H. W. Hirs and S. N. Timasheff, Eds. Academic Press, New York, 272-281 (1972).
Hsiung, et al., "Expression of Bovine Growth Hormone Derivatives in Escherichia coli and the Use of the Derivatives to Produce Natural Sequence Growth Hormone by Cathepsin C Cleavage, Methods in Enzymology", 153: 390-401 (1987).
G. T. Burke et al., Biochemical and Biophysical Research Communications, 173:3, 982-987 (1990).

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