Enzymatic reduction for producing optically active alcohols

Details Enzymatic reduction for producing optically active alcohols Enzymatic reduction for producing optically active alcohols

2011-02-15

2011-02-15

Ramirez, Delia M (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing heterocyclic carbon compound having only o, n, s,...

C435S195000

Reexamination Certificate

active

07888080

ABSTRACT:
A process for preparing optically active alkanols of the formula Iin whichn is an integer from 0 to 5;Cyc is an optionally substituted, mono- or polynuclear, saturated or unsaturated, carbocyclic or heterocyclic ring, andR1is halogen, SH, OH, NO2, NR2R3or NR2R3R4+X−, with R2, R3and R4independently of one another being hydrogen or a lower alkyl or lower alkoxy radical and X−being a counterion,which process comprises incubating in a medium comprising alkanone of the formula II in which n, Cyc and R1are as defined above,an enzyme having a polypeptide sequence(i) SEQ ID NO: 1 or(ii) in which, compared to SEQ ID NO:1, up to 25% of the amino acid radicals have been altered by deletion, insertion, substitution or a combination thereof and which retains at least 50% of the enzymic activity of SEQ ID NO:1,with the compound of the formula II being enzymically reduced to give the compound of the formula I, and isolating the product formed.

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