Enzymatic reagent system for total cholesterol assay using oxyge

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving cholesterol

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G01N 3114

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active

041614259

ABSTRACT:
A novel enzymatic reagent system is provided for assaying for total cholesterol in a liquid containing cholesterol, cholesterol esters, oxygen and substances which appear to inhibit the reactions between the enzymes and cholesterol and/or cholesterol esters. The novel reagent system comprises an aqueous solution of a buffering agent, cholesterol oxidase, cholesterol esterase, and a cationic surfactant in an amount sufficient to neutralize the effect of the inhibiting agents.

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