Enzymatic reactions and apparatus for carrying them out

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide

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4351731, 435183, 435219, C12N 2100

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active

056703325

ABSTRACT:
A water-supported enzymatic reaction is carried out with the substrate and the enzyme present in the solid phase. Either the water does not form a liquid continuum around substrate and enzyme, or the water acts at least partially out of the gas phase, or the water required for the reaction is added via a solid, or at least a part of the water required for the reaction is transferred by means of solid body contacts or by a combination of these means. The reaction mixture can be mixed by ultrasound or turbulence, e.g. in a fluidized bed, or both.

REFERENCES:
Kuhl, P., et al., Pharmazie 46:53 (1991).
C. H. Wong and K. T. Wang, "New Developments in Enzymatic Peptide Synthesis," Experientia, Dec. 1991, pp. 1123-1129.
"Biotechnology Techniques" vol. 6, No. 2 Mar./Apr. 1992 pp. 155-160.
Fulcrand-Rolland, et al., Biomed Biochim Acta 50:S213-S216 (1991).
Kuhl, P. et al., BBA 1078:326-8 (1991).

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