Enzymatic production of glycolic acid

Details Enzymatic production of glycolic acid Enzymatic production of glycolic acid

2008-09-15

2009-12-29

Ramirez, Delia M (Department: 1652)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S069100, C435S252300, C435S252330, C435S228000, C435S325000, C435S320100

Reexamination Certificate

active

07638617

ABSTRACT:
Various methods are provided for the enzymatic production of glycolic acid from glycolonitrile. These methods include: 1) use ofAcidovorax facilis72W nitrilase mutants having improved nitrilase activity for converting glycolonitrile to glycolic acid, and 2) methods to improve catalyst stability and/or productivity. The methods to improve catalyst stability/productivity include use of reaction stabilizers, running the reactions under substantially oxygen free conditions, and controlling the concentration of substrate in the reaction mixture.

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