Enzymatic production of difructose dianhydride IV from...

Details Enzymatic production of difructose dianhydride IV from... Enzymatic production of difructose dianhydride IV from...

2005-01-11

2005-01-11

Rao, Manjunath N. (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

C435S004000, C435S006120, C435S069100, C435S183000, C435S193000, C435S252300, C435S320100, C536S023200

Reexamination Certificate

active

06841368

ABSTRACT:
Disclosed is the production of difructose dianhydride IV from sugar. A sugar solution is subjected to reaction at room temperature or lower in an acidic buffer of pH 3.0-7.0 in the presence of a levansucrase derived fromZ. mobilisto produce levan. The levansucrase is prepared by culturingE. coliBL21 (DE3)/pEL12 (KCTC 8661), harvesting and homogenizing the cells, and isolating levansucrase from the cell homogenate. Levan is purified from the reaction solution and subjected to reaction at 25-50° C. for 3-10 hours in an acidic buffer of pH 3.0-7.0 in the presence of a levan fructotransferase to produce difructose dianhydride IV. The levan fructotransferase is obtained fromE. coliJUD81 (KCTC 0877BP). Also, disclosed are a gene coding for the levan fructotransferase and an expression vector pUDAF81 carrying the gene.

REFERENCES:
NIOC (GenBank Accession No. AAY04105 Jun. 10, 1999).*
Tanaka (a)et al. (J. Biochem., 1983, vol. 94(5):1569-1578).*
Tanaka (a)et al. (J. Biochem., 1985, vol. 97(6):1679-88).*
Song et al. (SPTREMBL Accession No. Q9KJD0, Oct. 1, 2000).*
Lee et al.,Federation of Microbiological Societies(FEMS)Microbiology Letters,vol. 195, pp. 127-132, 2001.
Song et al.,Enzyme and Microbial Technology,vol. 27, pp. 212-218, 2000.

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