Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1990-02-08
1992-03-10
Patterson, Charles L.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
435265, 8 9427, 210632, 210913, C12P 2106, C12S 700
Patent
active
050949462
ABSTRACT:
Materials containing both chromium and protein and treated by processes which include the steps of: combining a material to be treated (i.e. containing both chromium and protein) with sufficient water to produce a mixture having from about 75 wt. % water to about 95 wt. % water, and an additive which both provides an alkaline pH and provides calcium or magnesium as an enzyme cofactor. The mixture is thereafter held at a temperature of from about 60.degree. C. to about 75.degree. C. for a period of time of from about 0.5 hour to about 4 hours. Subsequently, at least one enzyme is utilized to hydrolyze the protein, thus producing a product containing solubilized hydrolyzed protein and insoluble chromium. After separation of the soluble fraction from the insoluble fraction, the chrome in the insoluble residue may be recycled into the pickling or tanning process, and the essentially chrome-free protein hydrolyzate is potentially useful as an ingredient in feeds, fertilizers, and cosmetics. The processes of the present invention are used to particular advantage to treat wastes from the leather tanning industry, for example, material such as blue stock, chrome shavings, chrome buffing dust, chrome cakes and chrome sludges.
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Taylor et al., J. Am. Leather Chem. Assoc. 81:4 (1986).
Iliskovic et al., Koza Obuca 34(6):130 (1985) (abstract).
Parvathi et al., Leather Sci., 31:236 (1984) (abstract).
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Parvathi et al., Leather Sci., 33:303 (1986).
Taylor, Maryann, et al., "Enzymatic Treatment of Solid Waste Generated in the Tanning Industry", Abstracts of IVLTCS, 20th Congress Oct. 15-19, 1989.
Taylor, Maryann M. et al., "Enzymatic Treatment of Chrome Shavings" JALCA vol. 84, p. 157, May 1989.
Abstract of Leder- und Hautemarkt., Gerbereiwissenschaft und Praxis, 30, 100 (1978).
Diefendorf Edward J.
Marmer William N.
Na George C.
Taylor Maryann M.
Patterson Charles L.
Sadowski David R.
Silverstein M. Howard
The United States of America as represented by the Secretary of
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