Enzymatic process for the determination of .beta.-lactam antibio

Details Enzymatic process for the determination of .beta.-lactam antibio Enzymatic process for the determination of .beta.-lactam antibio

1991-04-26

1993-09-21

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 23, 435 24, 435 29, 435184, 435810, 435825, 435975, 436 23, 436815, C12Q 100, C12N 100

Patent

active

052468305

ABSTRACT:
An enzymatic process for the determination of .beta.-lactam antibiotics in a biological liquid, comprising the steps of (1) incubating the liquid with soluble D-alanyl-D-alanine-carboxypeptidase produce by Actinomadura R39, the .beta.-lactam antibiotic reacting with the enzyme to form an inactive and equimolecular enzyme-antibiotic complex; (2) incubating the mixture obtained with a solution of a thioester substrate having the formula ##STR1## wherein R.sub.1 =benzoyl, phenylacetyl or N.sup..alpha. -acetyl-L-lysyl,

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Adam et al, Biochem J., vol. 270, pp. 525-529, 1990.
Lowe et al., Chemical Abstracts, vol. 63, No. 3 (1965), Abstract No. 3243e.

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