Enzymatic process for preparing [.gamma.-.sup.32 P]-labeled nucl

Details Enzymatic process for preparing [.gamma.-.sup.32 P]-labeled nucl Enzymatic process for preparing [.gamma.-.sup.32 P]-labeled nucl

1978-07-13

1980-06-24

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 92, 435148, 435147, C12D 1306

Patent

active

042095891

ABSTRACT:
ATP and other nucleoside triphosphates labeled in the .gamma.-phosphate with .sup.32 P are prepared from L-.alpha.-glycerophosphate and their corresponding nucleoside diphosphates by a series of enzymatic reactions in the presence of NAD.sup.+, preferably regenerated by lactate dehydrogenase and pyruvate. The resulting [.gamma.-.sup.32 P]nucleotides are useful as reagents for analytical determinations.

REFERENCES:
J. Biol. Chem. 248, 8319-8321 (1973).
Biochem. J. 90 147-149 (1964).

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