Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Patent
1996-10-09
1998-07-21
Saucier, Sandra E.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
435911, 435921, C12P 724
Patent
active
057834296
ABSTRACT:
A process for the enzymatic oxidation of alcohols to aldehydes in a continuous reaction series is disclosed utilizing crude cellular methanol oxidase and catalase produced by an acceptable organism such as those selected from the genera of Pichia, Hansenula, Candida and Torulopsis. The process maintains a steady-state aldehyde concentration whereby enzyme activity is promoted. In particular, the process has two reactors operating in series continuously producing a fermenter effluent containing growing, intact yeast cells at a cell concentration of about 0.5 to about 3% by weight of the effluent by continuous culture of the cells on a methanol carbon source, continuously introducing the effluent from the first reactor to the second reactor, adding an alcohol feed at about 0.2-10% w/w and oxygen to the second reactor, maintaining a steady-state aldehyde concentration in the reaction mixture, the concentration of residual alcohol being about 0.1-9% w/w by controlling the rates of introduction of effluent and alcohol feed.
REFERENCES:
patent: 4481292 (1984-11-01), Raymond
patent: 4720457 (1988-01-01), Armstrong et al.
patent: 4845033 (1989-07-01), Tegtmeier
patent: 4871669 (1989-10-01), Murray et al.
patent: 4900670 (1990-02-01), Zall et al.
patent: 4904590 (1990-02-01), Fukuda et al.
patent: 4920055 (1990-04-01), Hoiberg et al.
patent: 4962031 (1990-10-01), Yoshida et al.
patent: 5010005 (1991-04-01), Duff et al.
patent: 5182199 (1993-01-01), Hartley
patent: 5234827 (1993-08-01), Hatfield et al.
Duff S.J.B. et al., "Production and Application of Methylotrophic Yeast Pichia Pastoris", Biotech & Bioeng. 31: 44-49 (1988).
Kemp et al., "Inducible long chain alcohol oxidase from alkane-grown Candida tropicalis", Appl. Microbiol. Biotechnol. (1988) 29:370-374.
Murray et al., "Induction and stability of alcohol oxidase in the methylotrophic yeast pichia-pastoris.", Appl. Microbiol. Biotechnol. (1989) 32;95-100.
Duff et al., "Factors affecting the yeast-mediated conversion of ethanol to acetaldehyde in batch reactors", Enzyme Microb. Technol. (1989) 11:770-775 .
Gabelman Alan
Luzio Gary A.
Givaudan Roure Flavors Corporation
Saucier Sandra E.
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