Enzymatic modification of lecithin

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

Reexamination Certificate

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C435S132000, C435S155000, C435S041000, C554S080000, C426S534000, C426S442000, C426S478000

Reexamination Certificate

active

10822222

ABSTRACT:
The disclosure pertains to enzymatic modification of lecithin and to hydrolyzed lecithin products obtained by such modification. One particular implementation provides methods for producing a hydrolyzed lecithin product containing hydrolyzed phospholipids, monoglycerides, and diglycerides. For example, such a method may include the steps of: (a) contacting a lecithin material, which includes a phospholipid component and a triglyceride component, in an aqueous or organic solvent medium, with a first enzyme effective to hydrolyze the phospholipid; and (b) subsequently contacting the product of step (a) with a second enzyme, effective to hydrolyze the triglyceride; under reaction conditions effective to inhibit esterification of the hydrolyzed phospholipid with released fatty acids.

REFERENCES:
patent: 4584141 (1986-04-01), Paulitz et al.
patent: 4976984 (1990-12-01), Yasukawa et al.
patent: 5378623 (1995-01-01), Hattori et al.
patent: 6068997 (2000-05-01), Sas et al.
patent: 6773902 (2004-08-01), Chung et al.
patent: 2002/0122867 (2002-09-01), 't Hooft et al.
patent: 2003/0072856 (2003-04-01), Jirjis
patent: 2003/0175383 (2003-09-01), Bojsen et al.
patent: 713557 (1986-11-01), None
patent: WO-00/52190 (2000-09-01), None
patent: WO 2005/024036 (2005-03-01), None
VanMiddlesworth et al, J. Org. Chem., 1992, vol. 57, pp. 4753-4754.
Wikipedia, “Lecithin” http://en.wikipedia.org/wiki/Lecithin accessed on Oct. 4, 2005, 2 pages.
Garrett et al, “Biochemistry” 1999 Saunders College Publishing, Second Edition, pp. 242-247.
Hanahan et al, Journal of Biological Chemistry, 1960, vol. 235, No. 7, pp. 1917-1923.
Bornscheuer, U.T. “Lipase-catalyzed syntheses of monacylglycerols” Enzyme and Mcirobial Technology, 1995, vol. 17, pp. 578-586.
Bornscheuer, U.T., et al., “Optimizing lipases and related enzymes for efficient application,” Trends in Biotechnology, vol. 20, No. 10, Oct. 2002, pp. 433-437.
Kilcawley, K.N., et al., “Determination of key enzyme activities in commercial peptidase and lipase preparations from microbial or animal sources,” Enzyme and Microbial Technology, vol. 31, 2002, pp. 310-320.
Mustranta, A, et al., “Comparison of Lipases and Phospholipases in the Hydrolysis of Phospholipids,” Process Biochemistry, vol. 30, No. 5, 1995, Great Britain, pp. 393-401.
Haas, M.J., et al., “The Hydrolysis of Phosphatidylcholine by an Immobilized Lipase: Optimization of Hydrolysis in Organic Solvents,” Journal of the American Oil Chemists' Society, vol. 70, No. 2, Feb. 1993, pp. 111-117.
Haas, M.J., et al., “Enzymatic Phosphatidylcholine Hydrolysis in Organic Solvents: An Examination of Selected Commercially Available Lipases,” Journal of the American Oil Chemists' Society, vol. 71, No. 5, May 1994, pp. 483-490.
Haas, M.J., et al., “The Enzymatic Hydrolysis of Triglyceride-Phospholipid Mixtures in an Organic Solvent,” Journal of the American Oil Chemists' Society, vol. 72, No. 5, 1995, pp. 519-525.
Hara, F., et al., “Comparative Study of Commercially Available Lipases in Hydrolysis Reaction of Phosphatidylcholine,” Journal of American Oil Chemists' Society, vol. 74, No. 9, 1997, pp. 1129-1132.
Gunstone, F.D., “Enzymes as biocatalysts in the modification of natural lipids,” Journal of the Science of Food and Agriculture, vol. 79, No. 12, 1999, pp. 1535-1549.
Mustranta, A., et al., “Modification of Phospholipids With Lipases and Phospholipases,” Biocatalysis, vol. 9, 1994, pp. 181-194.
Pearce, S.W. et al., “Lipases for food ingredients”, 2002 Annual Meeting and Food Expo, Anaheim, California, XP-002337138, Session 94-5, 2002.
Pearce, S. et al., “Lipases for food ingredients”, XP-002337138 Internet Abstract 94-5, 2002 Annual Meeting and Food Expo, Anaheim, California.
Chrisope, G. and Marchall, R., “Combined Action of Lipase and Microbial Phospholipase C on a Model Fat Globule Emulsion and Raw Milk”,Journal of Dairy Science, 59(12):2024-2030, (1976).
Falcone, R. et al., “Effect of the Addition of a Nonaqueous Polar Solvent (Glycerol) on Enzymatic Catalysis in Reverse Micelles.Hydrolysis of 2-Naphthyl Acetate by—Chymotrypsin”,Langmuir20(14):5732-5737, (2004).
Gekko, K. and Timasheff, S., “Mechanism of Protein Stabilization by Glycerol: Preferential Hydration in Glycerol-Water Mixtures”,Biochemistry20(16):4667-4676, (1981).
Scow, R. and Egelrud, T., “Hydrolysis of Chylomichon Phosphatidylcholine In Vitro by Lipoprotein Lipase, Phospholipase A2and Phospholipase C”,Biochimica et Biophysica Acta431:538-549, (1976).

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