Enzymatic method to produce 7-dehydropregnenolone, vitamin...

Organic compounds -- part of the class 532-570 series – Organic compounds – 9,10-seco-cyclopentanohydrophenanthrene ring system or...

Reexamination Certificate

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Reexamination Certificate

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11031844

ABSTRACT:
Provided herein are enzymatic methods of producing steroid compounds such as 7-dehydropregnenolone, hydroxy derivatives thereof and vitamin D2- and D3-like compounds and derivatives thereof. Also provided are the derivatives of the vitamin D3-like compounds so generated via the action of cytochrome P450scc enzyme on substrates 7-dehydrocholesterol, vitamin D2 or vitamin D3.

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Shackleton et al., “Neonatal urinary steroids in Smith-Lemli-Optiz syndrome associated with 7-dehydrocholesterol reductase deficiency”, Steroids, vol. 64, pp. 481-490, 1999.
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Woods et al., “Expression of catalytically active human cytochrome P450scc inEscherichia coliand Mutagenesis of isoleucine-462”, Arch. Biochem. Biophys., fol 353(1), pp. 109-115, 1998.

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