Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1997-11-07
2000-03-14
Sisson, Bradley
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
435 691, 435 694, 514 1, 514 2, 530303, 530308, C12P 2106
Patent
active
060371439
ABSTRACT:
The method of the invention provides for the formation of a recombinant polypeptide which has been modified at the C-terminal end through the use of a transpeptidation process. The method is suitable for modifying recombinant polypeptides of any source including those which may be commercially available, those derived from recombinant single copy or multicopy polypeptide constructs, or those derived from single or multicopy recombinant fusion protein constructs. The transpeptidation reaction involves contacting an endopeptidase enzyme with a recombinant polypeptide to substitute an addition unit, of one or more amino acids, for a leaving unit, linked to a core polypeptide through a cleavage site recognized by the endopeptidase enzyme. Recombinant polypeptides derived from multicopy polypeptide constructs may be cleaved from the multicopy polypeptide at the N-terminal and C-terminal ends and simultaneously under go substitution of the leaving unit by the desired addition unit. The invention utilizes known and newly discovered cleavage recognition sites to effectuate the desired modification products.
REFERENCES:
patent: 4710382 (1987-12-01), Recker
patent: 5118666 (1992-06-01), Habener
patent: 5120712 (1992-06-01), Habener
patent: 5234820 (1993-08-01), Wagner et al.
patent: 5279954 (1994-01-01), Wagner et al.
patent: 5512459 (1996-04-01), Wagner et al.
patent: 5707826 (1998-01-01), Wagner et al.
Orskov et al. (1992) Diabetologia. Glucagon-like Peptide-1, A New Hormone of the Entero-insular Axis. 35:701-711.
Kempe et al. (1985) Gene. Multiple-copy Genes: Production and Modification of Monomeric Peptides from Large Multimeric Fusion Proteins. 39:239-245, 1985.
Gutniak et al. (1992) The New England Jounal of Medicine. Antidiabetogenic Effects of Glucagon-Like Peptide-1 (7-36) Amide in Normal Subjects and Patients with Diabetes Mellitus. 326:1316-1322.
Carter et al. (1990) American Chemical Society 0097-6156/90/0427. pp. 181-193.
Agarwal et al., Biochemical Pharm., 26. pp. 359-367 (1977).
Barker, Methods Enzymol., 34, pp. 317-328 (1974).
Bethell, et al., J. Biol. Chem., 254, pp. 2572-2574 (1979).
Bongers, et al., Biomed. Biochem. ACTA, 50, pp. S157-S162 (1991).
Canova-Davis, et al., Chemical Abstracts, Abstract No. 67959, vol. 115, No. 7: 429 (1991); see Abstract & Anal. Biochem., 196, No. 1, pp. 39-45 (1991).
Cha, et al., Biochemical Pharm., 24, pp. 2187-2197 (1978).
Cha, et al., Biochemical Pharm., 30, pp. 1507-1515 (1981).
Degrado et al., J. of Cellular Biochem., 29, pp. 83-93 (1985).
Engels, Protein Engineering, 1, pp. 195-199 (1987).
Gutniak, et al., New Eng. J. Med., 326, pp. 1316-1322 (1992).
Johansen, Carlsberg Res. Commun., 41, pp. 73-80 (1976).
Kempe, et al., Gene, 39, pp. 239-245 (1989).
Kempe, et al., Biotechnology, 4, pp. 565-568 (1992).
Kirshner, et al., J. Biotechnology, 12, pp. 247-260 (1989).
Marcus, Methods Enzymol., 34, pp. 377-385 (1974).
Matsumoto, Methods Enzymol., 34, pp. 329-341 (1974).
Matsuura et al., Methods Enzymol., 34, pp. 303-304 (1974).
Morihara, Trends in Biotechnology, 5, pp. 164-170 (1987).
Ohsuye et al., Biochem. Biophys. Res. Commun., 150, p. 1275 (1988).
Orskov, Diabetologia, 35, pp. 701-711 (1992).
Pierce et al., Biochem., 19, pp. 934-942 (1980).
Schellenberger et al., Int. J. Peptide Protein Res., 39, pp. 472-476 (1992).
Schellenberger et al., Ing. J. Peptide Protein Res., 41, pp. 326-332 (1993).
Scouten, Methods Enzymol., 34, pp. 288-294 (1974).
Shen, Proc. Natl. Acad. Sci. USA, 81, pp. 4627-4631 (1984).
Sutton et al., BBRC, 134, pp. 386-392 (1986).
Suzuki, et al., Chemical Abstracts, Abstract No. 91914, vol. 112, No. 11: Mar. 1990, & Endocrinology, 125, No. 6, pp. 3109-3114 (1989).
Taylor, P.W. et al., Biochemistry, 9, pp. 2638-2645 (1970).
Thorens, et al., Diabetes, 42, pp. 1219-1225 (1993).
Williams et al., Biochemical Pharm., 29, pp. 589-595 (1980).
Henriksen Dennis
Manning Shane
Partridge Bruce
Stout Jay
Wagner Fred W.
BioNebraska, Inc.
Longton Enrique D.
Sisson Bradley
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