Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1978-02-27
1980-12-23
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 4, C12Q 146
Patent
active
042411807
ABSTRACT:
A method for detecting the presence and quantitatively determining the amount of ionic or nonionic surfactants on a surface, particularly for the rapid determination of the surfactants on glass surfaces. The method comprises the use of an enzyme-substrate combination, including an enzyme which binds the ionic or nonionic surfactant on the surface, but is not deactivated, or inhibited, by the nonionic surfactant's binding. An indicator molecule is also present which is responsive to the product of the enzyme-substrate reaction. In operation, an aqueous solution of the enzyme and indicator is applied to the surface to be tested and sufficient time is allowed for any surfactant to dissolve and bind the enzyme. A solution of a standard ionic surfactant is then added which will bind and deactivate the enzyme if the enzyme has not been bound by any nonionic surfactant present. Finally, a solution of the enzyme substrate is added. Any color change in the test solution as a function of time, indicates the presence and concentration of the nonionic surfactant. To test for ionic surfactant, the step of addition of ionic surfactant may be omitted and the color change, if any, upon substrate addition is followed as a function of time.
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Keyes Melvin H.
Moore Garry L.
Click Myron E.
Kepplinger Esther M.
Nelson John R.
Owens--Illinois, Inc.
Wilson David H.
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