Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1992-08-12
1994-09-27
Kumar, Shailendra
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
435 701, 435 711, 435 41, 435175, 435182, 435185, 435284, 435285, 435286, 435288, 435280, 422149, 422236, 422238, 530344, 530801, 560 38, 560 40, 560 41, C12P 2100
Patent
active
053506812
ABSTRACT:
The present invention discloses a method for the enzymatic synthesis of a peptide. A protected peptide having a C-terminal carboxylate group or a protected, N-acyl amino acid having an alpha carboxylate group is reacted with a protected peptide having an N-terminal ammonium group or a protected amino acid having an alpha ammonium group in the presence of a condensation enzyme under conditions in which the carboxylate group and the ammonium group condense to form a protected, uncharged, peptide product. This peptide product is transported across a water-immiscible hydrophobic phase into an aqueous product phase and prevented from back diffusing across the water-immiscible hydrophobic phase. The peptide product can be converted, chemically or enzymatically, to a charged species that cannot back diffuse across the water-immiscible phase into the aqueous reaction phase. The water-immiscible hydrophobic phase is an ion rejection membrane separating the aqueous reaction phase from the product phase creating oil/water interfaces with each of the aqueous phases.
REFERENCES:
patent: 3625734 (1971-12-01), Ward, III
patent: 3779907 (1973-12-01), Li et al.
patent: 3813317 (1972-03-01), Benoiton et al.
patent: 3933781 (1976-01-01), Bachman
patent: 4119408 (1978-10-01), Matson
patent: 4119493 (1978-10-01), Isowa
patent: 4187086 (1980-02-01), Walmet et al.
patent: 4251631 (1981-02-01), Simon
patent: 4455210 (1984-09-01), Coker et al.
patent: 4622417 (1986-11-01), Barnett et al.
patent: 4670151 (1987-06-01), Bitter
patent: 4743547 (1988-05-01), Kitamura et al.
patent: 4786597 (1988-11-01), Matson et al.
patent: 4921612 (1990-05-01), Sirkar
patent: 4956289 (1990-09-01), Wiasidlo et al.
patent: 5002871 (1991-03-01), Iacobucci
patent: 5037741 (1991-08-01), Iacobucci
Liquid-Membrane Hollow Fiber Enzyme Reactors, Enzyme Engineering, vol. 3, pp. 19-28, (1978), Lo, et al.
Two Phase Biocatalytic Reaction, J. Chem. Tech. Biotechnol., vol. 32, 162-169, (1989), Lilly.
"Immobilized Lactose for Whey Hydrolysis Stability and Operating Strategy", Enzyme Engineering, vol. 4, pp. 67-76, Pitcher et al., (1978).
Coupling Separation and Enrichment to Chemical Conversion in a Membrane Reactor, Aiche Annual Meeting, Nov. 8-12, 1981, (Matson, et al.).
Peptide Synthetic Chemistry, pp. 53 to 59.
Matson, Membrane Reactors in Bioprocessing, Ann. N.Y. Acad. Sci., 469, 152 (1986).
Oyama et al., Synthesis of an Aspartame Precursor by Immobilized Thermolysin in an Organic Solvent, Journal of Organic Chemistry, vol. 46, 5241-5242 (1981).
Continuous Synthesis of N-(Benzyloxycarbonyl)-L-Aspartyl L-Phenylalanine Methyl Ester with Inmobolized Thermolysis in an Organic Solvent Biotechnology, vol. 3, 459-464, (May, 1985).
Kagaku Sosethsu, 35, 195 (1982), (Toyo Soda).
Chem. Abstracts, vol. 47, 8015g, (1953), Rabinovich, et al.
J. Biol. Chem., 1986, 221, (1970), Arvid, et al.
Can. J. Biochem., 48, 1058-1065, (1970), Purdier et al.
Use of N.alpha.-Formyl Protected Amino Acid Esters in Enzyme-Catalyzed Peptide Synthesis, Chemical Abstracts, vol. 113, 593-596, (1990), Floersheimer.
Brose Daniel J.
Iacobucci Guillermo A.
Ray Roderick J.
van Eikeren Paul
Kumar Shailendra
The Coca-Cola Company
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