Enzymatic ligation of 3'amino-substituted oligonucleotides

Details Enzymatic ligation of 3'amino-substituted oligonucleotides Enzymatic ligation of 3'amino-substituted oligonucleotides

1993-04-16

1997-01-14

Vogel, Nancy T.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 243, C12Q 168, C07H 2104

Patent

active

055938264

ABSTRACT:
Method and kits are provided for detecting one or more target nucleic acids. A first oligonucleotide having a 3' amino group and a second oligonucleotide having a 5' phosphate group are annealed to a contiguous complementary region of a target nucleic acid. Whenever the 3' terminal nucleotides of the first oligonucleotide and the 5' nucleotides of the second oligonucleotide are complementary to the opposing nucleotides on the target nucleic acid, a nucleic acid ligase ligates the first and second oligonucleotides via the formation of a phosphoramidate linkage. The presence of the target nucleic acid is determined by detection of the ligated first and second oligonucleotides.

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