Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1981-04-09
1982-10-26
Hruskoci, Peter A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 4, 435 7, 435 30, 23230B, C12Q 102
Patent
active
043562604
ABSTRACT:
Method for the determination of enzymatic activity in which a sample is added to a well in or a defined surface of a liquid-saturated porous matrix covering a thin layer of a substance sensitive to enzymatic lysis deposited on the solid surface of a carrier. The sample is permitted to diffuse or migrate electrophoretically in the matrix and react with the substance sensitive to enzymatic lysis. The matrix is then removed whereon lysis can be indicated on the solid surface. The enzyme-containing sample can be a bacteria colony on the matrix. Plastic material is preferred for the solid surface.
REFERENCES:
patent: 3401087 (1968-09-01), Kuzel
patent: 3730843 (1973-05-01), McKie
patent: 3905767 (1975-09-01), Morris et al.
patent: 3917515 (1975-11-01), Goldberg
patent: 4066512 (1978-01-01), Lai et al.
patent: 4169765 (1979-10-01), Keyes
patent: 4234683 (1980-11-01), McMillan
Webster, "Deoxyribonuclease: A Sensitive Assay Using Radial Diff. in Agarose Containing Methyl-Green-DNA Complex", Biochem. Biophys. Acta, 247 (1971), pp. 54-61.
Chemical Abstracts, 77 (1972), 30705r.
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