Enzymatic immunological method for the determination of antigens

Details Enzymatic immunological method for the determination of antigens Enzymatic immunological method for the determination of antigens

1975-09-04

1977-04-05

Monacell, A. Louis

Textiles: fiber preparation

195127, 195 99, C12K 104

Patent

active

040160432

ABSTRACT:
The present invention relates to improvements in the sandwich technique for the determination of a component of an antigen-antibody reaction in a liquid sample to be tested, utilizing as reagents (a) one component of said reaction bound to the surface of a water-insoluble, water-insuspensible, solid carrier, and (b) a component having the same immunological properties covalently linked to an enzyme. The liquid sample is contacted and incubated with the reagent(s) to form a reaction mixture, the enzyme activity of either the liquid or solid phase of which is a measure of the presence and quantity of the component to be determined. The method is especially useful for diagnostic testing for hepatitis or rubella antibodies.

REFERENCES:
patent: 3791932 (1974-02-01), Schums et al.
patent: 3852157 (1974-12-01), Rubenstein et al.
patent: 3876504 (1975-04-01), Koffler
patent: 3896217 (1975-07-01), Johnson
patent: 3905767 (1975-09-01), Morris et al.

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