Enzymatic immunoassay involving detecting fluorescence while osc

Details Enzymatic immunoassay involving detecting fluorescence while osc Enzymatic immunoassay involving detecting fluorescence while osc

1992-05-20

1993-08-24

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 79, 435 21, 435808, 436526, 436534, 436800, 436805, C12Q 142

Patent

active

052388158

ABSTRACT:
Enzymatic immunoassay for estimating the concentration of antigen or antibody comprising bringing an antigen-antibody complex, which is labelled by an enzyme and is present heterogeneously in a solution, into contact with a substrate where pH of the solution is so adjusted as to be suitable for the enzyme to be activated and also for measuring the fluorescence of the substrate, measuring the time-variation of fluorescent intensity of the substrate produced by the enzyme reaction, and estimating the concentration of the antigen or the antibody from the slope of the substantially linear portion, on a characteristic curve representing variation of the fluorescence intensity with the time.
Fluorescence is measured while magnetic beads with antigen-antibody complex attached are oscillated at a specific frequency.

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patent: 4604364 (1986-08-01), Kosak
patent: 4614713 (1986-09-01), Harnisch
patent: 4659657 (1987-04-01), Harnisch
Yolken: Reviews of Infectious Diseases 4(1) pp. 35-45 and 50, (1982).
Tietz, N. W., ed, Fundamentals of Clinical Chemistry, W. B. Saunders Company, Philadelphia, pp. 373-375 (1970).

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