Enzymatic fluorometric assay for adenylate cyclase

Details Enzymatic fluorometric assay for adenylate cyclase Enzymatic fluorometric assay for adenylate cyclase

1994-01-21

1997-04-08

Gitomer, Ralph

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 21, 435963, C12Q 100

Patent

active

056186657

ABSTRACT:
A method for measuring the amount of adenylate cyclase without the use of radioactive reagents is provided. The method comprises combining a sample of physiological material containing an amount of cAMP with (a) a mixture of enzymes effective to eliminate any other endogenous adenine nucleotides which may be present in the sample; and (b) an amount of alkaline phosphatase effective to eliminate any glucose-6-phosphate present in the sample. The cAMP present in said sample is then converted to AMP and the amount of AMP measured, which may then be correlated to the amount of cAMP and AC present in the sample.

REFERENCES:
patent: 5312810 (1994-05-01), Wood et al.
patent: 5316907 (1994-05-01), Lurie et al.
Proc. Natl. Acad. Sci. USA, Vol. 78, No. 4, issued Apr., 1981. Rossomando et al., "Formycin 5'-triphosphate, a Fluorescent Analog of ATP, as a Substrate for Adenylate Cyclase", pp. 2278-2282.
Journal of Chromatography, vol. 400, issued 1987, Yoshioka et al., "Analyses of Adenosine and Adenine Nucleotides inBiological Materials by Fluorescence Reaction-High-Performance Liquid Chromatography", pp. 133-144.
Journal of Cyclic Nucleotide Research, vol. 7, No. 1, issed 1981, Wojcik et al., "A Simple Fluorometric Method of cAMP" Application to Studies of Brain Adenylate Cylcase Activity, pp. 27-35.

Affiliated with

Also associated with

Rating

Enzymatic fluorometric assay for adenylate cyclase does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enzymatic fluorometric assay for adenylate cyclase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzymatic fluorometric assay for adenylate cyclase will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2397075