Enzymatic fluorometric assay for adenylate cyclase

Details Enzymatic fluorometric assay for adenylate cyclase Enzymatic fluorometric assay for adenylate cyclase

1993-01-22

1994-05-31

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 19, 435 21, 435 25, 435191, 435195, 435963, 435968, 436 63, 436172, 436805, 436811, C12Q 100, G01N 2176

Patent

active

053169077

ABSTRACT:
A method of measuring adenylate cyclase (AC) in a sample of physiological material which does not employ radioactive reagents is provided, comprising:

REFERENCES:
M. M. Bradford et al., "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding", Anal. Biochem., 72, 248 (1976).
McL. Breckenridge, "The measurement of cyclic adenylate in tissues", PNAS USA, 52, 1580 (1964).
M. R. Bristow et al., "Decreased catecholomaine sensitivity and B-adrenergic-receptor density in failing human hearts", New Engl. J. Med., 307, 205 (1982).
M. R. Bristow et al., "Histamine-mediated adenylate cyclase stimulation in human myocardium", Mol. Pharmacol., 21, 671 (1982).
N. D. Goldberg et al., "Enzymatic analysis of cyclic 3', 5'-AMP in mammalian tissues and urine", Anal. Biocheml, 28 523 (1969).
E. Heimreich et al., "The effects of pH and temperature on the kinetics of the phosphorylase reaction", Proc. Natl. Acad. Sci. (US), 52(3), 647-54 (1964).
E. Heimreich et al., "The role of adenylic acid in the activation of phosphorylase", Proc. Natl. Acad. Sci. (US), 51(1) 131-8 (1964).
C. L. Johnson et al., "Studies on histamine H.sub.2 receptors coupled to cardiac adenylate cyclase", Mol. Pharmacol., 16, 417 (1979).
O. H. Lowry et al., A Flexible System of Enzymatic Analysis, Academic Press, New York, pp. 129-218 (1972).
O. H. Lowry et al., "Effects of adenylic acid on the kinetics of muscle phosphorylase a", J. Biol. Chem., 239, 1947 (1964).
K. G. Lurie et al., "Increased B-adrenergic receptor density in an experimental model of cardiac transplantation", J. Thorac. Cardiovasc. Surg., 86, 195 (1983).
K. G. Lurie et al., "Metabolism and electrophysiology in subendocardial Purkinje fibers after infraction", Am. J. Physiol., 253, H662-H670 (1987).
F. M. Matschinsky et al., "Quantitative histochemical analysis of glycolytic intermediates and cofactors with an oil well technique", J. Histochem. Cytochem., 16, 29 (1968).
K. J. Rocha-Singh, et al., "Hypoxia and glucose independently regulate the B-adrenergic receptor-adenylate cyclase system in cardiac myocytes", J. Clinical Investigation, 88, 204 (1991).
Y. Salomon et al., "A highly sensitive adenylate cyclase assay", Analy. Biochem., 58, 541 (1974).
Y. Salomon et al., "Adenylate Cyclase Assay", Adv. Cyclic Nucleotide Res., 10, 35 (1979).
C. Sungman et al., "Kinetics of cyclic enzyme systems", Mol. Pharmacol., 1, 178 (1965).
M. Trus et al., "Effects of glucose on insulin release and on intermediary metabolism of isolated perifused pancreatic islets form fed and fasted rats", Diabetes, 29, 1 (1980).
Rossomando et al., Proc. Natl. Acad. Sci. USA, vol. 78, No. 4, pp. 2278-2282, Apr. 1981.
Yoshioka et al., Journal of Chromatography, vol. 400, pp. 133-141, 1987.

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