Enzymatic DNA molecules that contain modified nucleotides

Drug – bio-affecting and body treating compositions – Enzyme or coenzyme containing – Hydrolases

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 911, 435 9131, 435183, 435196, 435199, 435 9153, 424 941, 424 9461, 536 231, 536 245, C07H 2104, C12Q 168, A61K 3846, C12N 922, C12P 1934

Patent

active

061104620

ABSTRACT:
The present invention discloses catalytic or enzymatic DNA molecules that contain a modified nucleotide and that are capable of cleaving nucleic acid sequences or molecules, particularly RNA, in a site-specific manner, as well as compositions including same. Methods of making and using the disclosed enzymes and compositions are also disclosed.

REFERENCES:
Barbas & Sakthivel, Expanding the potential of DNA for binding and catalysis: Delineation of a class of highly functionalizated dUTP derivatives that are substrates for thermostable DNA polymerases, Angewandte Chemie, vol. 37. No. 20, pp. 2872-2875, Oct. 1998.
Yu, et al., "Cyanine dye dUTP analogs for enzymatic labeling of DNA probes", Nucleic Acids Research 22 (15): 3226-3232 (1994).
Kovacs, et al., "Simple Synthesis of 5-Vinyl- and 5-Ethynyl-2'-Triphosphates", Tetrahedron Letters 29 (36): 4525-4528 (1998).
Langer, et al., "Enzymatic synthesis of biotin-labeled polynucleotides: Novel nucleic acid affinity probes", Proc. Natl. Acad. Sci. USA 78: USA 78: 6633-6637 (1981).
Cook, et al., "Synthesis and hybridization of a series of biotinylated oligonucleotides", Nucleic Acids Research 16 (9): 4077-4095 (1988).
Aurup, et al., 2'-Fluoro- and 2'-Amino-2'-deoxynucleoside 5'-Triphosphates as Substrates for T7 RNA Polymerase, Biochemistry 31 (40): 9636-9641 (1992).
Limbach, et al., "Summary: the modified nucleosides of RNA", Nucleic Acids Research 22 (12): 2183-2196 (1994).
Dewey, et al., "The RNA World: Functional Diversity in a Nucleoside by Carboxyamidation of Uridine", Nucleosides& Nucleotides l5 (10): 1611-1617 (1996).
Dewey, et al., "New Uridine Derivatives for Systematic Evolution of RNA Ligands by Exponential Enrichment", J. Am. Chem. Soc. 117: 8474-8475 (1995).
Crouch, et al., "Synthesis of 2'-Deoxyuridine Nucleosides with Appended 5-Position Carbonyl Cross-Linking Groups", Nucleosides & Nucleotides 13 (4): 939-944 (1994).
Eaton, et al., "Ribonucleosides and RNA", Annu. Rev. Biochem. 64: 837-863 (1995).
Lorsch, et al., "Chance and Necessity in the Selection of Nucleic Acid Catalysts", Acc. Chem. Res. 29: 103-110 (1996).
Breaker, "In Vitro Selection of Catalytic Polynucleotides", Chem. Rev. 97: 371-390 (1997).
Jaschke, et al., "In Vitro Selected Oligonucleotides as Tools in Organic Chemistry", Synlett 6: 825-833 (1999).
Tarasow, et al., "Characteristics of an RNA Diels--Alderase Active Site", J. Am. Chem. Soc. 121: 3614-3617 (1999).
Rawls, "Catalytic DNAs", C&EN (Feb. 3, 1997) Science/Technology, pp. 33-35.
Eaton, et al., "Let's get specific: the relationship between specificity and affinity", Chemistry & Biology 2 (10): 633-638 (1995).
Williams, et al., "Function of specific 2'-hydroxyl groups of guanosines in a hammerhead ribozyme probed by 2' modifications", Proc. Natl. Acad. Sci. USA 89: 918-921 (1992).
Santoro, et al., "Mechanism and Utility of an RNA-Cleaving DNA Enzyme", Biochemistry 37: 13330-13342 (1998).
Sakthivel, et al., "Expanding the Potential of DNA for Binding and Catalysis: Highly Functionalized dUTP Derivatives That Are Substrates for Thermostable DNA Polymerase", Angew. Chem. Int. Ed. 37 (20): 2872-2875 (1998).
Santoro, et al., "A general purpose RNA-cleaving DNA enzyme", Proc. Natl. Acad. Sci. USA 94: 4262-4266 (1997).
Breaker, et al., "A DNA enzyme that cleaves RNA", Chemistry & Biology 1: 223-229 (1994).
Joyce, "Nucleic acid enzymes: Playing with a fuller deck", Proc. Natl. Acad. Sci. USA 95: 5845-5847 (1988).
delCardayre, et al., "Structural Determinants of Enzymatic Processivity", Biochemistry 33 (20): 6031-6037 (1994).
Pyle, "Ribozymes: A Distinct Class of Metalloenzymes", Science 261: 709-714 (1993).
Symons, "Ribozymes", Current Opinion in Structural Biology 4: 322-330 (1994).
Joyce, "In vitro evolution of nucleic acids", Current Opinion in Structural Biology 4: 331-336 (1994).
Breaker, et al., "Inventing and improving ribozyme function: rational design versus iterative selection methods", Tibtech 12: 268-275 (1994).
Joyce, "Amplification, mutation and selection of catalytic RNA", Gene 82: 83-87 (1989).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Enzymatic DNA molecules that contain modified nucleotides does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enzymatic DNA molecules that contain modified nucleotides, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzymatic DNA molecules that contain modified nucleotides will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1246698

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.