Enzymatic DNA molecules

Details Enzymatic DNA molecules Enzymatic DNA molecules

2006-11-28

2006-11-28

Zara, Jane (Department: 1635)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S006120, C435S091100, C435S091310, C536S023100

Reexamination Certificate

active

07141665

ABSTRACT:
The present invention discloses deoxyribonucleic acid enzymes—catalytic or enzymatic DNA molecules—capable of cleaving nucleic acid sequences or molecules, particularly RNA, in a site-specific manner, as well as compositions including same. Methods of making and using the disclosed enzymes and compositions are also disclosed.

REFERENCES:
patent: 5861288 (1999-01-01), Usman et al.
patent: 5879938 (1999-03-01), Usman et al.
patent: 6008343 (1999-12-01), Jennings et al.
patent: 6159714 (2000-12-01), Usman et al.
patent: 6361941 (2002-03-01), Todd et al.
patent: WO95/11304 (1995-04-01), None
Agrawal et al. Molecular Medicine Today, 2000, vol. 6, p. 72-81.
Opalinska et al. Nature Reviews Drug Discovery, 2002, vol. 1, p. 503-514.
Jen et al. Stem Cells 2000, vol. 18, p. 307-319.
Breaker, DNA enzymes, 1997,Nature Biotech., 15:427-431.
Beaudry and Joyce, “Directed Evolutionof an RNA Enzyme”,Science, 257:635-641 (1992).
Breaker, et al., “Continuous in Vitro Evolution of Bacteriophage RNA Polymerase Promoters”,Biochemistry, 33:11980-11986 (1994).
Breker and Joyce, “Inventing and Improving Ribozyme Function: Rational Design Versus Iterative Selection Methods”,Trends Biotech, 12:268-275 (1994).
Breaker and Joyce, “A DNA Enzyme with Mg2+Dependent RNA Phosphoesterase Activity”,Chemistry&Biology, 2:655-660 (1995).
Burgstaller and Famulok, “Synthetic Ribozymes and the First Deoxyribozyme”,Angew. Chem. Int. Ed. Engl., 34:1189-1192 (1995).
Cadwell and Joyce, “Randomization of Genes by PCR Mutagenesis”,PCR Methods and Applications, 2:28-33 (1992).
Cadwell and Joyce, “Mutagenic PCR”,PCR Methods and Applications, 3(Suppl): s136-s140 (1994).
Chartrand, et al., “A Oligodeoxyribonucleotide with Catalytic Properties”,Proc. RNA Society, (1994) Abstract.
Joyce and Inoue, “A Novel Technique for the Rapid Preparation of Mutant RNAs”,Nucleic Acids Research, 17:711-722 (1989).
Joyce, “Amplification, Mutation and Selection of Catalytic RNA”,Gene, 82:83-87 (1989).
Joyce, “In Vitro Evolution of Nucleic Acids”,Current Opinion in Structural Biology, 4:331-336 (1994).
Lehman and Joyce, “Evolution In Vitro of an RNA Enzyme with Altered Metal Dependence”,Nature, 361:182-185 (1993).
Pan and Uhlenbeck, “In Vitro Selection of RNAs that Undergo Autolytic Cleavage with Pb2+”,Biochemistry, 31:3887-3895 (1992).
Pan and Uhlenbeck, “A Small Metalloribozyme with a Two-Step Mechanism”,Nature, 358:560-563 (1992).
Paquette, et al., “The Conformation of Single-Stranded Nucleic Acids tDNA Versus tRNA”,Eur. J. Biochem., 189:259-265 (1990).
Perreault, et al., “Mixed Deoxyribo- and Ribo-oligonucleotides with Catalytic Activity”,Nature, 344:565-567 (1990).
Robertson and Joyce, “Selection In Vitro of an RNA Enzyme that Specifically Cleaves Single-Stranded DNA”,Nature, 344:467-468 (1990).
Tsang and Joyce, “Evolutionary Optimization of the Catalytic Properties of a DNA-Cleaving Ribozyme”,Biochemistry, 33:5966-5973 (1994).
Williams, et al., “Function of Specific 2′-Hydroxyl Groups of Guanosines in a Hammerhead Ribozyme Probed by 2′ Modifications”,Proc. Natl. Acad. Sci., USA, 89:918-921 (1992).
Yang, et al., “Minimum Ribonucleotide Requirement for Catalysis by the RNA Hammerhead Domain”,Biochemistry, 31:5005-5009 (1992).

Affiliated with

Also associated with

Rating

Enzymatic DNA molecules does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enzymatic DNA molecules, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzymatic DNA molecules will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3683935