Enzymatic diagnostic composition

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving glucose or galactose

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435 4, 435 15, 435 17, 435 21, 435 26, 435181, C12Q 100, C12Q 150, C12N 1106

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active

043716113

ABSTRACT:
Disclosed herein is a method for binding enzymes comprising reacting either a synthetic or a natural polymer containing carboxyl and amino groups with an excess of a urethane prepolymer followed by coupling of an enzyme thereto by use of a carbodiimide. The product of the process is also described as well as the use thereof in analyzing blood fluids. The invention is particularly adapted to binding hexokinase and glucose-6-phosphate dehydrogenase to determine creatine phosphokinase (CPK) in human blood.

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Mosbach et al., "Matrix-bound Enzymes I, Use of Different Acrylic Copolymers as Matrixes," Chem. Abstracts, vol. 74, No. 5, p. 48 (1971), Absts. No. 19777h.

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