Enzymatic diagnostic composition

Details Enzymatic diagnostic composition Enzymatic diagnostic composition

1980-09-23

1983-02-01

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving glucose or galactose

435 4, 435 15, 435 17, 435 21, 435 26, 435181, C12Q 100, C12Q 150, C12N 1106

Patent

active

043716113

ABSTRACT:
Disclosed herein is a method for binding enzymes comprising reacting either a synthetic or a natural polymer containing carboxyl and amino groups with an excess of a urethane prepolymer followed by coupling of an enzyme thereto by use of a carbodiimide. The product of the process is also described as well as the use thereof in analyzing blood fluids. The invention is particularly adapted to binding hexokinase and glucose-6-phosphate dehydrogenase to determine creatine phosphokinase (CPK) in human blood.

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Mosbach et al., "Matrix-bound Enzymes II Matrix-bound Two-enzyme System," Chem. Abstracts, vol. 74, No. 5, p. 48, (1971), Absts. No. 19778i.
Mosbach et al., "Matrix-bound Enzymes I, Use of Different Acrylic Copolymers as Matrixes," Chem. Abstracts, vol. 74, No. 5, p. 48 (1971), Absts. No. 19777h.

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