Enzymatic diagnostic composition

Details Enzymatic diagnostic composition Enzymatic diagnostic composition

1978-08-07

1980-10-07

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving glucose or galactose

435 4, 435 15, 435 17, 435 21, 435 26, 435181, C12Q 100, C12Q 150, C12N 1106

Patent

active

042269350

ABSTRACT:
Disclosed herein is a method for binding enzymes comprising reacting an amino acid and/or a protein with an excess of a urethane prepolymer, curing the resulting product and coupling an enzyme thereto by use of a carbodiimide. The product of the process is also described as well as the use thereof in analyzing blood fluids. The invention is particularly adapted to binding hexokinase and glucose-6-phosphate dehydrogenase to determine creatine phosphokinase (CPK) in human blood serum.

REFERENCES:
patent: 3524062 (1971-04-01), Sato
patent: 3705084 (1972-12-01), Reynolds
patent: 3838011 (1974-09-01), Hagen et al.
patent: 3905923 (1975-09-01), Klug
patent: 3928138 (1975-12-01), Wood et al.
patent: 3929574 (1975-12-01), Wood et al.
patent: 3933594 (1976-01-01), Milligan et al.
patent: 4094744 (1978-06-01), Hartdegen et al.
patent: 4095948 (1978-06-01), Hunziker
patent: 4098645 (1978-07-01), Hartdegen et al.

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