Enzymatic determination of theophylline

Details Enzymatic determination of theophylline Enzymatic determination of theophylline

1991-12-31

1993-02-23

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 25, 435 26, 435191, 435832, C12Q 144

Patent

active

051889412

ABSTRACT:
The present invention provides a new methodology and test composition for determining the presence of theophylline in test samples. The methodology employs enzymes that utilize or recognize theophylline as a substrate to measure the concentration thereof in samples, including body fluids. This new approach utilizes enzymes as opposed to traditional methods which use antibodies for the recognition of theophylline. The enzymatic approach to theophylline determination is quick, simple and convenient and allows test systems to be made in liquid as well as in dry-chemistry formats. Various protocols, systems or methodologies may be used for assaying and relating the results to the amount of theophylline present. Methods for obtaining theophylline utilizing or recognizing enzymes are also described.

REFERENCES:
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patent: 4341868 (1982-07-01), Nakanishi et al.
Bhargava et al., Engymatic Theophylline Assay . . . , 41st National Meeting of the Am. As. for Clinical Chem., Jul. 23-27, 1989 Clin. Chem. 35(6) 1989 p. 1102.
Gupta et al., A Novel Enpymatic Approach for Serum Theophylline Measurement, 40th Nat. Meeting of the Am. As for Clinical Chem. Jul. 24-28, 1988 Clin. Chem. 34(6) 1988 p. 1267.
Bergomeyer, Methods of Enzymatic Analysis, 3rd ed. vol. III, Xanthine Oxidase p. 211, 1983.
R.A. Robson et al. Characterization of Theophliline Metabolism in Human Liver Microsomes Brit. J. of Clin Pharm. (1987) 24, 293-300.

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